可听的声波通过机械刺激促进了附着癌细胞的EV形成和分泌
Zhuoyue Lei1,2, Hongwei Jiang3, Jie Liu1,2
1College of Biomedical Engineering, Sichuan University, Chengdu 610065, China.
ACS applied materials & interfaces
|November 1, 2023
概括
听觉声波 (AAW) 显著提高癌症衍生的细胞外囊泡 (EV) 产量高达2.5倍. 这种新的方法可以提高癌症转移研究的EV产量,而不会改变EV的质量或功能.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 来自癌症的细胞外囊泡 (EVs) 在转移研究中至关重要.
- 目前用于EV生物制造的方法效率低下,限制了大规模研究.
研究的目的:
- 开发一种用于增强癌症衍生的EV生物制造的新方法.
- 调查可听声波 (AAW) 对电动汽车生产和特性的影响.
主要方法:
- 利用可听的声波 (AAW) 对癌细胞施加机械刺激.
- 使用常规静态培养与AAW刺激的量化EV产量.
- 分析了EV形态,大小,泽塔潜力和功能能力 (迁移,入侵).
- 研究了潜在的分子机制,包括ESCRT通路和相关蛋白质.
主要成果:
- 与静态培养相比,AAW在3天内将癌症衍生的EV产量提高了2.5倍.
- 通过AAW刺激的电动汽车保持了与传统生产的电动汽车相比较的形态,尺寸和zeta潜力.
- 通过AAW刺激的EVs有效地促进了2D和3D培养中的癌细胞迁移和入侵.
- 与ESCRT通路激活和膜融合蛋白的上调相关的EV生产增强.
结论:
- AAW是一种有效的方法,用于高数量,高质量的EV生物制造.
- 这种方法有可能促进基于电动汽车的研究和治疗应用.
- AAW可以增强来自各种细胞类型的EV生产,支持更广泛的科学和翻译努力.
相关概念视频
01:13Cell-matrix's Response to Mechanical Forces
2.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
2.7K
01:35Cancer Cell Migration through Invadopodia
2.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.3K
01:26Tension Response at Adherens Junctions
2.7K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.7K