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德尔塔的Exopalaemon carinicauda:分子特征,不同组织和发育阶段的表达,以及其SNP与发育发展的关联分析

Xiaofang Lai^1,2,3,4, Fanyue Ji⁵, Feifan Yu⁵

¹Jiangsu Key Laboratory of Marine Bioresources and Environment, Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang, 222005, Jiangsu, China. lai.xiaofang@163.com.

Molecular biology reports

|November 1, 2023

概括

德尔塔基因对Exopalaemon carinicauda的发展至关重要,影响卵巢成熟和胚胎发育. 三角洲的遗传变异 (SNP) 与繁殖成功有关,为的分子繁殖提供了潜力.

科学领域:

海洋生物学海洋生物学
发展生物学发展生物学
遗传学遗传学是一个

背景情况:

痕信号通路对于许多生物体的发育至关重要.
在Exopalaemon carinicauda的发展中,Delta的特定作用仍然在很大程度上是未知的.

研究的目的:

研究德尔塔在Exopalaemon carinicauda发育中的分子特征和功能.
探索三角洲基因变异与发育特征的关联.

主要方法:

快速放大cDNA末端 (RACE) 用于基因测序.
定量实时PCR (qRT-PCR) 用于基因表达分析.
RNA干扰 (RNAi) 来评估基因功能.
单核酸多态性 (SNP) 分析以确定遗传变异.

主要成果:

在E. carinicauda中确定了Delta的全长cDNA,显示了与其他物种的高度同源性.
德尔塔表达在卵巢中最高,并且在不同发育阶段有所不同.
德尔塔RNA干扰显著降低了诺奇通路基因表达,并延迟了卵巢发育.
德尔塔基因中的特定SNP,特别是那些影响EGF类域的SNP,与Gonadosomatic Index (GSI) 的显著差异相关.

结论:

德尔塔在Exopalaemon carinicauda的发展中发挥着重要作用.
在三角洲发现的SNP为旨在改善的发育和繁殖的繁殖计划提供了潜在的分子标记物.

关键词:

国家统一计划与发展之间的关联克隆和表达方式德尔塔德尔塔德尔塔是什么意思埃克索帕莱蒙卡里尼卡乌达 (Exopalaemon carinicauda) 是一个非常古老的植物.这是一种RNA干扰.

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