临床应用的质谱分析:一篇综述
Hong Zhang1, Yali Yang1,2, Yanxiao Jiang1
1School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, P. R. China.
Critical reviews in analytical chemistry
|November 1, 2023
概括
质谱 (MS) 为临床分析提供了高灵敏度和特异性,超过了传统方法. 未来的发展,如微型仪器和临床检测,有望在医疗保健中得到更广泛的应用.
科学领域:
- 临床分析化学 临床分析化学
- 质谱仪的应用
- 生物医学诊断 生物医学诊断
背景情况:
- 质谱法 (MS) 在临床环境中越来越受欢迎,因为它的灵敏度,特异性和吞吐量优越.
- 像液体染色学-并联质谱学 (LC-MS/MS) 这样的技术比免疫测试具有明显的优势,使其成为黄金标准.
- 目前的审查侧重于MS在临床研究和诊断中的实用性和进展.
研究的目的:
- 总结质谱在临床分析中的关键特征和各种应用.
- 突出基于MS的方法在临床研究中的效率和优势.
- 探索用于临床环境的质谱技术的未来前景和潜在进展.
主要方法:
- 审查有关质谱技术及其临床应用的现有文献.
- 分析与MS检测相结合的分离技术,如LC-MS/MS.
- 探索环境电离质谱 (AIMS) 在临床环境中的潜力.
主要成果:
- 质谱仪表现出高灵敏度,特异性和吞吐量,使其成为临床分析的理想选择.
- 在许多临床应用中,LC-MS/MS已经成为黄金标准,在许多临床应用中表现优于免疫测试.
- 环境电离质谱 (AIMS) 显示出由于样品预处理的最小程度和增强的流量而有前途.
结论:
- 质谱法是临床分析和研究的强大工具,与传统方法相比,它具有显著的优势.
- 未来的方向包括小型化,护理点测试和高通量分析,以扩大MS采用范围.
- 艾姆斯是一个显著的进步,可能简化临床工作流程和扩大MS实用.
相关概念视频
MALDI-TOF Mass Spectrometry
4.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.8K
Mass Spectrometry: Complex Analysis
796
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
796
Mass Spectrometry: Overview
5.3K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.3K
Peptide Identification Using Tandem Mass Spectrometry
6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K
Mass Spectrometers
5.6K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.6K


