原生体Aurantiochytrium具有普遍的亚端粒rDNA,是米鲁病毒的宿主
Jackie L Collier1, Joshua S Rest2, Lucie Gallot-Lavallée3
1School of Marine and Atmospheric Sciences, Stony Brook University, Nicolls Road, Stony Brook, NY 11794, USA.
Current biology : CB
|November 1, 2023
概括
在丰富的海洋真核生物Aurantiochytrium limacinum中发现了类似于疹病毒的海洋真核病毒. 这项研究使用长读测序将这些病毒与它们的宿主联系起来,揭示了独特的基因组元素.
科学领域:
- 海洋病毒学 海洋病毒学
- 欧核生物基因组学
- 分子生物学分子生物学
背景情况:
- 病毒在海洋中丰富,发挥着关键的生态作用.
- 转基因组学揭示了新的真核病毒,包括神经病毒,通常缺乏宿主识别.
- 疹类型的病毒颗粒以前在海洋迷宫菌体中观察到,但仍然没有表征.
研究的目的:
- 为了将米鲁病毒的生物学与它们的甲状腺宿主联系起来.
- 为了描述海洋真核生物Aurantiochytrium limacinum中的新型病毒元素.
- 为了研究A. limacinum的基因组配置和病毒关联.
主要方法:
- 长时间阅读的Aurantiochytrium limacinumATCC MYA-1381.1.的基因组测序
- 对染色体结构的分析,包括分端体区域和重复元素 (LORE-TEARS).
- 病毒基因组元素的识别和表征 (CE1插曲和LE-Chr15集成元素).
主要成果:
- A. limacinum的基因组由26个线性染色体组成,具有独特的亚端粒rDNA和LORE-TEARS配置.
- 确定了两种与mirusvirus相关的基因组元素:一个副本数量高的插曲 (CE1) 和一个集成的元素 (LE-Chr15).
- 这些元素与疹病毒和核细胞质大DNA病毒 (NCLDVs) 有相似之处,这表明了米鲁病毒和螺杆菌之间的联系.
结论:
- 这项研究确立了mirusvirus和thraustochytrids之间的联系,以Aurantiochytrium limacinum为例.
- 已识别的病毒元素 (CE1和LE-Chr15) 类似于疹病毒的病例性和内源性潜伏形式.
- 这些发现为海洋病毒与宿主相互作用以及大型DNA病毒的演化开辟了新的研究途径.
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