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DNA损伤诱导的自受Candida albicans中的内醇多酸盐合成酶调节
Jiawen Du1, Yixuan Dong1, Hangqi Zhu1
1Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Biochimica et biophysica acta. Molecular cell research
|November 1, 2023
概括
在Candida albicans中发现了DNA损伤诱导的自. 内醇多酸盐,特别是Kcs1和Vip1,对于这个过程至关重要,影响关键的自和DNA修复基因.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 由DNA损伤引起的自是一种与宏自不同的过程,在Candida albicans中以前没有被发现.
- 伊诺西多酸盐调节DNA修复和宏自,但它们在DNA损伤引起的自中所起的作用尚不清楚.
研究的目的:
- 为了识别和描述Candida albicans中DNA损伤诱导的自.
- 研究伊诺西聚酸盐途径在调节这一过程中的作用.
主要方法:
- 对C. albicans菌株缺乏关键的伊诺西聚酸盐合成酶 (Kcs1,Vip1) 的遗传分析.
- 显微镜检查法戈聚集点 (PAS) 和自体.
- 分析与自相关的基因1 (Atg1) 招募和拉帕素复合物1 (TORC1) 酶活性的目标.
- 对DNA损伤反应基因的基因表达分析,如RAD53.
主要成果:
- 由DNA损伤引起的自需要C. albicans的核心宏观自机制.
- Kcs1和Vip1对于高效的DNA损伤诱导的自是必不可少的,影响PAS和自体的形成.
- 缺少Kcs1或Vip1会损害Atg1的招募,增加TORC1的活动,改变Atg18的局部化,并降低RAD53.
结论:
- 在Candida albicans中存在DNA损伤诱导的自.
- 通过Kcs1和Vip1合成的伊诺西酸对于调节C. albicans中DNA损伤诱导的自和DNA修复途径至关重要.
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