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在与年龄相关的卵巢储量减少时,kaempferol通过调节HSP90/NRF2通路来发挥抗氧化作用

Zhoujia Hua¹, Wei Zhang¹, Lin Han¹

¹Department of Reproduction, Zhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, China.

Chemical biology & drug design

|November 1, 2023

概括

凯姆菲罗尔 (Kaempferol) 是Er-Xian脱的关键成分,通过减少氧化应激来缓解与年龄相关的卵巢储量减少 (AR-DOR). 它抑制HSP90的表达,从而提高NRF2的调节,为AR-DOR提供了潜在的治疗策略.

科学领域:

生殖生物学生殖生物学
药理学药理学是指药理学的学科.
氧化压力研究研究氧化压力研究

背景情况:

与年龄相关的卵巢储量减少 (AR-DOR) 是一个临床挑战.
含有kaempferol的Er-Xian脱液 (EXD) 用于治疗卵巢功能障碍.
在AR-DOR中使用凯姆菲罗尔的机制尚不清楚.

研究的目的:

阐明kaempferol在治疗AR-DOR中的机制.
研究HSP90和NRF2通路在kaempferol作用中的作用.
在AR-DOR中为kaempferol的临床应用提供理论基础.

主要方法:

与DOR患者和志愿者进行的临床研究.
AR-DOR小鼠模型用kaempferol,GGA或GDA进行治疗.
试验包括ELISA用于激素和氧化应激标志物,H&E染色用于AFC,以及西班牙涂抹蛋白质水平 (HSP90,NRF2,p-HSF1,p-Src).

主要成果:

在AR-DOR小鼠中,卡姆菲洛尔治疗改善了血清AMH,E2,AFC,SOD和CAT水平,同时降低了FSH,ROS和MDA.
凯姆菲洛尔抑制了HSP90表达和上调NRF2表达.
GGA逆转了kaempferol的作用,而GDA则增强了它们,这表明HSP90抑制的作用.

结论:

在AR-DOR中,kaempferol通过抑制HSP90和上调NRF2.2来产生抗氧化作用.
这种机制为在治疗AR-DOR时使用kaempferol提供了科学理由.
这些发现支持kaempferol作为与年龄相关的卵巢储量减少的潜在治疗剂.

关键词:

埃尔-西安的蒸.与年龄相关的卵巢储备减少.热冲击蛋白质 90 90 90 90 90 90 90 90 90 热冲击蛋白质是什么?热冲击蛋白质是什么?凯姆菲罗尔醇是什么凯姆菲罗尔核因子红色素2相关因子2的核因子.

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