含有新型水溶性芳香保护组的氨基酸结合物的自组合和细胞相容性
Valeria Castelletto1, Lucas de Mello1,2, Emerson Rodrigo da Silva2
1School of Chemistry, Food Biosciences and Pharmacy, University of Reading, Whiteknights, Reading RG6 6AD, United Kingdom.
Biomacromolecules
|November 1, 2023
概括
新的2,7-disulfo-9-fluorenylmethoxycarbonyl (Smoc) 保护的氨基酸可以自组装成纳米结构. 这些Smoc-氨基酸显示出出色的生物相容性和细胞吸收,显示出生物材料的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 生物化学 生物化学
背景情况:
- 由于其自组装特性和生物相容性,受到9 - 烯甲基 (Fmoc) 保护的具有兴趣.
- 新型的2,7-二硫-9-甲甲基 (Smoc) 保护组使水基合成成为可能.
- 斯莫克-氨基酸结合物为先进的生物材料提供了潜在的潜力.
研究的目的:
- 研究Smoc结合氨基酸 (Smoc-Ala,Smoc-Phe,Smoc-Arg) 的自我组装行为和生物相容性.
- 描述由此产生的纳米结构和形成的二次结构.
- 为了评估Smoc-氨基酸的细胞吸收和细胞毒性.
主要方法:
- 合成斯莫克-氨基酸合物.
- 低温传输电子显微镜 (Cryo-TEM) 和小角度X射线散射 (SAXS) 用于结构分析.
- 提奥夫拉T (ThT) 光,循环二极化 (CD) 和富里埃变形红外光谱 (FTIR) 用于二次结构的确定.
- 光测试以确定临界聚合度 (CAC).
- 在纤维细胞中进行细胞吸收研究的共聚焦显微镜.
- 细胞毒性测试用于评估纤维细胞代谢和生存能力.
主要成果:
- 斯莫克氨基酸自组装成纳米片,纳米丝带或纳米管,在它们的临界聚合度 (CAC) 以上.
- 光谱分析证实了组装的中β-叶子二次结构的形成.
- 斯莫克的内在光允许CAC的确定和可视化细胞吸收到纤维细胞质的细胞质.
- 观察到很好的生物相容性,没有细胞毒性,甚至在低度下增强了纤维细胞的新陈代谢.
结论:
- 斯莫克氨基酸能够形成各种纳米结构,具有β-叶子二次动机.
- 斯莫克的自我光性质有助于表征和成像.
- 斯莫克氨基酸具有很高的生物相容性和细胞吸收,表明它们对生物相容材料和细胞培养中的应用具有前景.
相关概念视频
Peptide Bonds
74.6K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
74.6K
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
3.0K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...
3.0K
Amino acids
89.0K
Amino acids are the monomers that comprise proteins. Each amino acid has the same fundamental structure, which consists of a central carbon atom, or the alpha (α) carbon, bonded to an amino group (NH2), a carboxyl group (COOH), and to a hydrogen atom. Every amino acid also has another atom or group of atoms bonded to the central atom known as the R group. There are 20 common amino acids present in proteins, each with a different R group. Variation in the amino acid sequence is responsible...
89.0K
Phase II Reactions: Sulfation and Conjugation with α-Amino Acids
256
Sulfation and α-amino acid conjugation are two critical biotransformation reactions in drug metabolism. Sulfation, a phase II biotransformation reaction, involves adding a polar sulfate group to a drug, enhancing its water solubility and promoting excretion. This process can either co-occur with or occur independently of glucuronidation. Nonmicrosomal sulfotransferase enzymes catalyze the process. The reaction involves 3'-phosphoadenosine-5'-phosphosulfate or PAPS coenzyme...
256


