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Gli1标记了一种用于肌肉再生的哨兵肌肉干细胞群

Jiayin Peng¹, Lili Han², Biao Liu¹

¹State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, PR China.

Nature communications

|November 2, 2023

概括

通过Gli1表达识别的肌肉干细胞 (MuSCs) 的特定子集对于有效的肌肉再生至关重要. 这些Gli1阳性MuSCs更活跃,对受伤迅速作出反应,启动修复过程.

科学领域:

肌肉干细胞生物学肌肉干细胞生物学
骨肌肉的再生和再生
细胞异质性细胞异质性

背景情况:

成年骨肌的再生依赖于肌肉干细胞 (MuSCs).
已知 MuSCs 是异质的.
在MuSC中存在不同的功能子集的存在尚未完全理解.

研究的目的:

调查异质群体中是否存在一个独特的MuSC子集.
描述潜在的Gli1表达MuSC子集的功能和特性.
确定Gli1+ MuSCs在肌肉再生中的作用.

主要方法:

对表达Gli1 (Gli1+ MuSCs) 的MuSCs的鉴定.
在体外和体内测试以评估Gli1+ MuSCs的增殖和分化.
耗尽研究评估Gli1+ MuSCs对肌肉再生的影响.
在未受伤和受伤的肌肉中对 Gli1+ 和 Gli1- MuSC 的比较分析.
对mTOR信号,细胞大小,线粒体含量和GAlert细胞形成的分析.

主要成果:

确定了Gli1+ MuSCs的种群,并发现它们对于肌肉再生至关重要.
Gli1+ MuSCs表现出增强的扩散和分化能力.
Gli1+ MuSCs的耗尽延迟了肌肉再生;Gli1+ MuSCs的移植更有效.
在未受伤的肌肉中,Gli1+ MuSCs显示了增强的mTOR信号传递,更大的细胞大小和更多的线粒体,表明了原始状态.
Gli1+ MuSCs对受伤后的GAlert细胞形成有显著的贡献.

结论:

Gli1+ MuSCs代表了一种独特的,原始化的MuSC群体,在平静肌肉中具有增强的活性.
这个Gli1+ MuSC子集作为哨兵群体,在受伤时迅速进入细胞循环,以启动骨肌肉再生.

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