相关实验视频
Updated: Jun 29, 2026
11:19
Silk Film Culture System for in vitro Analysis and Biomaterial Design
Published on: April 24, 2012
19.5K
来自Bombyx mori子的素的净化和干燥方法
Ana Paula Sone1, Marcelino Luiz Gimenes2, Jaqueline Hoscheid3
1Department of Chemical Engineering, State University of Western Paraná, Toledo, Brazil.
Natural product research
|November 2, 2023
概括
该研究发现,冷/解净化和喷雾干燥是有效的,可持续的方法,可以生产无污染物的血. 这些技术为先进的材料开发提供了经济和环境的好处.
科学领域:
- 生物材料科学 生物材料科学
- 可持续化学 可持续化学
- 织工程 织工程 织工程
背景情况:
- 丝加工中的蛋白质素 (Sericin) 通常含有污染物.
- 开发可持续和安全的素净化方法对于其更广泛的应用至关重要.
- 现有的净化方法可能不环保或经济可行.
研究的目的:
- 评估不同净化和干燥方法的sericin.
- 确定可持续和经济上有利的技术,以生产无污染物的血.
- 为了比较通过各种方法获得的素的结构和物理性质.
主要方法:
- 使用酒精溶剂和冷解周期净化素.
- 使用冷化和喷雾原子化干燥净化的血清素.
- 对素产量的分析,结构性质 (β-sheets,随机卷,β-turns),光度,可溶性和粒子形态的分析.
主要成果:
- 在净化方法之间没有观察到显著的产量差异.
- 结冰解净化证明了经济和环境的优势.
- 与冷化颗粒相比,喷雾干燥的素颗粒更发光,更可溶,更球形.
- 这两种干燥方法都产生了具有不同结构构造的无形素颗粒.
结论:
- 通过冷解和通过喷雾原子化干燥的净化适合获得没有污染物的素.
- 这些方法与开发更安全和可持续的环境技术保持一致.
- 选择的方法提供了效率,成本效益和环境责任的平衡.
相关概念视频
01:18Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
01:30Methods of Sterilization II: Chemical Methods
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...