巴西棕色及其二烯的莱什曼活动
Larissa Costa Oliveira1, Victor Pena Ribeiro1, Mario Ferreira Conceição Santos1
1Research Center in Exact Sciences and Technologies, University of Franca, Franca, São Paulo, Brazil.
Natural product research
|November 2, 2023
概括
巴西棕色提取物 (HEBP) 有效地对抗Leishmania寄生虫,显示出显著的抗寄生虫活性. 然而,从提取物中分离出来的迪特尔在本研究中没有显示出雷什曼杀伤性.
科学领域:
- 自然产品 化学 化学
- 寄生虫学的寄生虫学
- 药用化学 医学化学
背景情况:
- 是一种天然树脂混合物,由于其多样化的生物活性,在传统医学中被使用.
- 巴西丰富的生物多样性有助于其大量生产各种类型.
- 蜂的抗寄生素特性特别有趣,特别是对于像莱什曼病这样的疾病.
研究的目的:
- 为了研究巴西棕色 (HEBP) 的醇提取物的杀性潜力.
- 评估HEBP中特定的分离烯对Leishmania的活性.
- 描述HEBP对Leishmania寄生虫形态学的影响.
主要方法:
- 制备和提取巴西棕色 (HEBP).
- 从HEBP中分离和识别了六种主要的二烯.
- 在实验室中评估了针对*Leishmania* (*Leishmania*) *amazonensis*前列腺炎的莱什曼杀菌活性.
- 显微镜技术 (TEM和SEM) 用于观察寄生虫的结构变化.
主要成果:
- 巴西棕色 (HEBP) 的水醇提取物表现出强烈的杀性活性,IC50为8.32μg/mL.
- 在测试度下,没有任何一种分离的二甲烯 (酸,13-epi-cupressic酸,13-epi-torulosol,脱水酸,cis-communic酸和ent-agatic酸) 在测试度下表现出显著的活性.
- HEBP治疗导致了L*的显著形态和结构变化. (*L.*) *amazonensis*促进性菌,通过TEM和SEM观察到的情况.
结论:
- 巴西棕色 (HEBP) 的水醇提取物是开发针对莱什曼病的新治疗方法的有希望的候选者.
- HEBP的莱什曼杀伤作用可能是由于其他化合物而不是测试的二烯.
- 对HEBP的活性成分及其作用机制的进一步研究是有必要的.
相关概念视频
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Leishmaniasis is a protozoal disease caused by species of the genus Leishmania and transmitted through the bite of infected female sandflies. The parasite exists in two principal morphological forms during its life cycle. A sandfly acquires intracellular amastigotes from an infected reservoir host, such as a dog. Within the sandfly, these forms differentiate into motile, flagellated promastigotes. During a subsequent blood meal, promastigotes are injected into the human host, where they...
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Leishmaniasis is a widespread parasitic disease caused by several Leishmania species. It affects millions of people each year and remains a major public health problem in endemic regions. First-line treatment relies on pentavalent antimonials, including meglumine antimoniate and sodium stibogluconate. Even so, how these drugs work has not been fully clear, especially their interaction with parasite-specific biochemical pathways. One key target is trypanothione reductase (TR), an enzyme that...