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Updated: Jul 12, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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使用CRISPR-Cas9基的细胞素和腺素基编辑器编辑Drosophila的生殖系编辑

Nirav Thakkar1,2, Adela Hejzlarova1, Vaclav Brabec1

  • 1Biology Center of the Academy of Sciences of the Czech Republic, Institute of Entomology, Ceske Budejovice, Czech Republic.

The CRISPR journal
|November 2, 2023
PubMed
概括

基编辑器在果 (Drosophila melanogaster) 中有效地实现了生殖基因编辑,在28°C的温度下性能最佳. 这项研究还产生了无时代基因的新等位基因,影响昼夜节律.

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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