在Trypanosoma cruzi epimastigotes中,形态发生过程是通过高度不对称的细胞分裂进行的
Paul C Campbell1, Christopher L de Graffenried1
1Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island, United States of America.
PLoS neglected tropical diseases
|November 2, 2023
概括
特里帕诺osoma cruzi epimastigote 细胞分裂是高度不对称的,产生不平等的子细胞. 这项研究揭示了在这个研究不足的寄生虫阶段显著的形态蛋白质局部化和分裂机制.
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 热带疾病 热带疾病
背景情况:
- 克鲁兹杆菌 (Trypanosoma cruzi) 导致查加斯病,这是一个被忽视的热带疾病.
- 在其生命周期中,T. cruzi表现出细胞两极分化和形态变化.
- 试类动物的细胞分裂机制是已知的,但对于T. cruzi epimastigotes的情况则不太清楚.
研究的目的:
- 为了研究昆虫居住的Trypanosoma cruzi. epimastigote形式的细胞分裂机制.
- 为了确定与相关的trypanosomatids相比,形态蛋白质定位的差异.
- 了解形态差异对寄生虫分裂的影响.
主要方法:
- 对形态基因的基于Cas9的标记.
- 活细胞成像 活细胞成像
- 扩展显微镜.扩展显微镜.
主要成果:
- 在T. cruzi epimastigote细胞分裂是高度不对称的,产生一个较小的子细胞.
- 观察到子细胞分裂速率 (4.9小时) 的显著差异.
- 与T. brucei. 相比,Morphogenic蛋白在T. cruzi epimastigotes中显示出改变的局部化模式.
结论:
- T. cruzi的表皮虫分裂不同于其他试虫的生命阶段,涉及细胞体的扩大/缩短而不是延长.
- 改变的蛋白质定位表明在这个阶段有独特的分裂机制.
- 形态变异显著影响了trypanosomatid寄生虫的分裂.
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