对有机细胞DNA的大规模入侵推动了毒素的核基因组进化
Sivaranjani Namasivayam1, Cheng Sun2, Assiatu B Bah2
1Department of Genetics, University of Georgia, Athens, GA 30602.
概括
东菌的基因组不断获得有机细胞起源的核DNA (NUOT),包括线粒体 (NUMTs) 和塑 (NUPTs) 序列. 这个过程显著塑造了寄生虫的形状.
科学领域:
- 基因组学就是基因组学.
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
背景情况:
- 毒素菌是一种广泛传播的动物性寄生虫,影响了三分之一的人类.
- 这种寄生虫拥有核,塑体 (ptDNA) 和线粒体 (mtDNA) 基因组.
- 对于理解寄生虫进化和适应而言,物种内基因变异至关重要.
研究的目的:
- 调查T. gondii基因组中细胞器DNA (NUOT) 的核整合物的程度和影响.
- 确定NUMT和NUPT收购的机制和进化意义.
- 探索NUOTs在基因调节和寄生虫适应中的功能作用.
主要方法:
- 对T. gondii ME49的基因组测序和分析.
- 与Neospora caninum进行比较的基因组学.
- 克里斯普尔-卡斯9诱导的双链断裂和安普利康序列.
- 报告员试图评估NUMTs.T的 cis调节功能.
主要成果:
- 在T. gondii ME49核基因组中,NUOT占1.6%,是报告中最高的比例.
- NUMT和NUPT是持续获得的,这对遗传变异有很大的贡献.
- 有证据表明,NUMT的移动和固定早于Toxoplasma和Neospora的分歧.
- 很大一部分NUMT位于基因内或附近,其中一些具有cis-regulatory活性.
结论:
- 器官细胞DNA整合是一个动态的过程,塑造T. gondii基因组.
- 保存的NUMT表明了进化约束和潜在的功能角色.
- NUOT可能有助于这种主要的人类病原体的适应性和表型多样性.
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