Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

SAM-dependent methyltransferases: from chemoenzymatic natural product synthesis to industrial application.

Natural product reports·2026
Same author

Rising tree mortality in France is associated with distinct seasonal climate anomalies.

Nature communications·2026
Same author

Creating molecular complexity in the chemoenzymatic synthesis of chlorothricin analogues using tandem Diels-Alderases.

Organic & biomolecular chemistry·2026
Same author

Crystal Structure and Conformational Dynamics of N─N Bond-Forming Piperazate Synthase.

Chembiochem : a European journal of chemical biology·2026
Same author

Substrate scope of ancestral versus modern family-1 glycosidases.

Protein science : a publication of the Protein Society·2026
Same author

High-throughput assays for SAM-dependent methyltransferases: advances, challenges, and future perspectives.

Natural product reports·2026

相关实验视频

Updated: Jul 11, 2025

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.6K

一种针对SAM依赖的甲基转移酶的通用,连续的检测方法.

Marian J Menke1, Pascal Schneider2, Christoffel P S Badenhorst1

  • 1Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Str. 4, 17487, Greifswald, Germany.

Angewandte Chemie (International ed. in English)
|November 2, 2023
PubMed
概括

我们开发了一种高通量试验,用于选S-adenosyl-l-methionine依赖甲基转移酶 (MTs). 这种酶测定能够有效地识别甲基转移酶,用于药物发现和开发.

关键词:
酶催化酶的催化作用高通量选 高通量选同类半氨酸同类半氨酸.硫化是一种硫化.在SAM-依赖的甲基转移酶中.

更多相关视频

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.3K
A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
08:17

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

2.4K

相关实验视频

Last Updated: Jul 11, 2025

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.6K
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.3K
A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
08:17

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

2.4K

科学领域:

  • 生物化学 生物化学
  • 酶学 是一种酶学.
  • 药物发现 药物发现 药物发现

背景情况:

  • 酶催化后期功能化 (LSF) 提供了一条通往强效活性药物成分 (API) 的途径.
  • 依赖S-adenosyl-l-methionine的甲基转移酶 (MTs) 进行选择性甲基化,超越了传统的化学方法.
  • 识别合适的MT对于推进API开发至关重要.

研究的目的:

  • 开发一种基于持续光的高通量试验,用于选SAM-依赖的甲基转移酶.
  • 为了能够有效地识别和表征甲基转移酶,用于制药应用.

主要方法:

  • 一种新的两步酶定量测试被设计为S-adenosyl-l-homocysteine转化为H2S.
  • 使用阿齐多库马林硫化物探针实现了选择性光读取.
  • 该试验使用表达O-MTs和N-MTs的大肠杆菌细胞溶解物进行了验证.

主要成果:

  • 开发的试验成功确定了E. coli细胞溶解物中的甲基转移酶活性.
  • 该试验证明了SAM依赖甲基转移酶的高通量选的适用性.
  • 研究了两个O-MT和一个N-MT,证实了测试性能.

结论:

  • 基于光的测定方法有效地识别和表征甲基转移酶.
  • 这种高通量方法通过LSF促进了新API的发现.
  • 该试验通过利用酶性甲基化来支持开发更强效的候选药物.