相关实验视频

Updated: Jul 11, 2025

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate NMDA Receptor in Blood

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate NMDA Receptor in Blood

Published on: January 9, 2018

化学自身抗体受体T细胞消耗NMDA受体特异性B细胞

S Momsen Reincke¹, Niels von Wardenburg¹, Marie A Homeyer²

¹Department of Neurology and Experimental Neurology, Charité - Universitätsmedizin Berlin, Berlin, Germany; German Center for Neurodegenerative Diseases (DZNE) Berlin, Berlin, Germany; Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Berlin, Germany.

|November 2, 2023

概括

研究人员开发了特定于NMDAR的仿制性自身抗体受体 (CAAR) T细胞以向自身免疫脑炎. 这些工程T细胞选择性地消除产生有害自身抗体的B细胞, 为NMDAR脑炎提供了有前途的新疗法.

科学领域:

免疫学
神经科学
细胞治疗

背景情况:

抗NMDA受体 (NMDAR) 自体是NMDAR脑炎的主要原因,这是一种严重的自身免疫神经疾病.
目前对NMDAR脑炎的治疗包括广泛的免疫抑制或非特异性抗体去除,这可能导致显著的副作用和复发.

研究的目的:

使用工程T细胞开发一种针对NMDAR脑炎的新型治疗方法.
创建NMDAR特异性仿制性自身抗体受体 (NMDAR-CAAR) T细胞,用于选择性消除致病性B细胞和自身抗体.

主要方法:

通过将细胞外的NMDAR自身抗原与细胞内信号域 (4-1BB/CD3ζ) 融合而设计的NMDAR-CAAR T细胞.
评估NMDAR- CAAR T细胞功能,包括识别患者衍生的自身抗体,效应分子释放,增殖和杀目标细胞.
在NMDAR脑炎的被动转移小鼠模型中评估了NMDAR- CAAR T细胞的疗效和安全性.

主要成果:

NMDAR-CAAR T细胞有效地识别了广泛的人类抗NMDAR自身抗体.
工程T细胞表现出强大的效应器功能,包括选择性杀死抗原特异性细胞,即使在高自身抗体度.
在体内研究显示,抗NMDARB细胞减少,自身抗体水平持续下降,但没有显著的异位毒性.

结论:

对于NMDAR脑炎,NMDAR- CAAR T细胞是一种高度特异且有效的临床前治疗策略.
这种有针对性的方法有可能减少治疗副作用,预防疾病复发,并改善患者的治疗结果.
临床前的成功支持NMDAR- CAAR T细胞治疗进入临床试验的NMDAR脑炎和其他自身免疫性疾病.

关键词:

一个CAAR T细胞抗NMDA受体脑炎一个T细胞自体免疫性脑炎自体免疫性细胞治疗嵌合体自身抗体受体

更多相关视频

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

Published on: August 21, 2017

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Published on: December 1, 2023

相关概念视频

Diversity of Antigen Receptors

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...