微生物分层保护脱的厌氧甲氧化古生物和细菌免受膜生物膜反应器中的外部氧气冲击
Pan-Long Lv1, Chuan Jia1, Xu Guo2
1Key Lab of Environmental Engineering, Shaanxi Province, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China.
Bioresource technology
|November 2, 2023
概括
溶解的氧度梯度会影响微生物群落,并结合去化无氧甲氧化 (DAMO) 和无氧氧化 (Anammox) 过程. 生物膜分层保护DAMO细菌免受氧气冲击,而Anammox细菌则被取代.
科学领域:
- 环境微生物学 环境微生物学
- 废水处理 废水处理
- 生物地质化学循环的过程
背景情况:
- 无氧甲氧化 (DAMO) 和无氧氧化 (Anammox) 的化过程对水生环境中的去除至关重要.
- 了解环境因素,如溶解氧 (DO) 对这些微生物群落的影响,对于优化废水处理过程至关重要.
研究的目的:
- 为了研究不同溶解氧 (DO) 梯度对微生物群体结构和去除途径的作用,结合DAMO-Anammox工艺在一个批量生物膜反应堆.
- 阐明微生物在不同氧气条件下的适应和弹性机制.
主要方法:
- 使用一批生物膜反应器来模拟不同的溶解氧 (DO) 水平.
- 监控的酸盐去除速度和积累.
- 通过分子技术评估微生物社区的组成.
- 量化了参与DAMO和Anammox通路的关键功能基因 (mcrA,hzo) 的丰富性.
主要成果:
- 完全无氧的条件促进了有效的酸盐去除 (72毫克NO3--N/L),而0.5毫克/L的DO水平导致酸盐积累峰值 (95毫克NO3--N/L).
- 0.2 mg/L的DO水平显著降低了关键的DAMO细菌 (Candidatus Methylomirabilis,Candidatus Methanoperedens) 和Anammox细菌 (Candidatus Kuenenia) 的丰度,降至5%以下.
- 关键基因mcrA (逆甲基生成) 和hzo (无氧氨氧化酶) 的表达在高DO下降.
结论:
- 溶解的氧气梯度极大地调节了DAMO-Anammox合过程.
- 氧冲击会导致微生物种群的转变,甲类植物和脱剂可能会在外部生物膜层中取代Anammox细菌.
- 生物膜微生物分层为DAMO细菌和古生物提供了对外部氧气暴露的保护机制,保持了过程完整性.
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