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Lukas Bernauer¹, Paula Berzak¹, Leonie Lehmayer¹

¹Institute of Molecular Biotechnology, Graz University of Technology, NAWI Graz, Graz, Austria; BioTechMed-Graz, Graz, Austria.

Journal of lipid research

|November 2, 2023

概括

细胞表面传感器 Wsc1-3 需要特定的 ergosterol 结合才能正常工作. 用胆固醇取代厄戈斯特醇会破坏细胞壁完整性的信号传递.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
微生物学微生物学

背景情况:

Wsc1,Wsc2和Wsc3是关键的细胞表面传感器.
这些蛋白在细胞壁应激时激活细胞壁完整性通路 (CWIP).

研究的目的:

为了研究固醇在Wsc蛋白功能中的作用.
为了确定胆固醇是否可以替代ergosterol在Wsc介导的信号中.

主要方法:

在K. phaffii*中对固醇生物合成的基因操纵.
对Slt2酸化和细胞壁生物合成的分析.
使用光显微镜进行蛋白质局部化研究.
网站导向的Wsc蛋白的突变发生.
用光反应性固醇衍生物进行生物化学分析.

主要成果:

局部胆固醇的产生导致Slt2的高酸化和细胞壁生物合成的上调.
删除Wsc基因可以逆转这些胆固醇诱导的表型.
胆固醇与 Wsc 跨膜域中的 CRAC 固醇结合基因相互作用.
在CRAC基因中的突变破坏了固醇结合.
与哺乳动物整合素观察到类似的胆固醇相互作用.

结论:

Wsc 传感器的功能取决于特定的 ergosterol 结合.
胆固醇不能有效地替代 Wsc 信号中的 ergosterol.
跨膜域中的CRAC图案对固醇相互作用和Wsc功能至关重要.

关键词:

细胞壁的完整性细胞壁的完整性科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.在MAPK信号传输中.它们是WSC蛋白质.固醇结合基因的基因是固醇.

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