孕期暴露在颗粒物中的神经发育后果 10:使用贝叶斯方法进行超声波发声和基因表达分析
Diego Ruiz-Sobremazas1, Mario Ruiz Coca2, Miguel Morales-Navas2
1Department of Psychology, Health Research Center (CEINSA), Almeria University, 04120, Almeria, Spain; University of Zaragoza, Department of Psychology and Sociology, Teruel, Spain.
Environmental research
|November 2, 2023
概括
孕期暴露于颗粒物 (PM) 通过大鼠的口服增加了幼犬的超声波发声 (USV). 这种暴露也改变了大脑中的基因表达,这表明空气污染和神经发育变化之间存在联系.
科学领域:
- 环境健康 环境健康
- 神经科学是一个神经科学.
- 毒理学 毒理学 毒理学
背景情况:
- 空气污染与心脏呼吸系统疾病有关,新出现的证据表明与神经发育障碍有关.
- 环境污染物的暴露途径,如颗粒物 (PM),通常通过吸入进行研究,但口服暴露也与此相关.
- 关于口服PM暴露及其对大脑发育的影响的临床前研究有限.
研究的目的:
- 为了研究口服孕期暴露于颗粒物 (PM) 对大鼠幼的超声波发声 (USV) 的影响.
- 为了分析在妊娠期间口服PM暴露后大脑中的基因表达变化.
主要方法:
- 怀孕的老鼠在整个怀孕期间都被口服暴露于颗粒物 (PM).
- 记录和分析幼的超声波发声 (USV).
- 用定量PCR分析幼大脑中的基因表达.
主要成果:
- 与对照组相比,在孕期暴露于PM的小鼠幼发出了更多的USV呼叫.
- 暴露的幼的USV持续时间更长,并且更早地开始.
- 暴露在PM下调了大脑中的Gabrg2和Maoa基因的表达,影响GABAergic和单氨基系统.
结论:
- 口服妊娠期暴露于PM10可能会影响神经发育,可能与社会行为或其他USV可以检测到的现象有关.
- 观察到的基因表达变化表明神经传递系统的改变.
- 需要进一步的研究来阐明空气污染物暴露对神经发育障碍和神经传递的全部影响.
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