相关实验视频

Updated: Jul 31, 2026

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Published on: October 17, 2011

在现场量化活体胚胎组织内的透压

Antoine Vian^1,2, Marie Pochitaloff^1,2, Shuo-Ting Yen^1,2

¹Department of Mechanical Engineering, University of California, Santa Barbara, CA, USA.

Nature communications

|November 3, 2023

概括

我们开发了新的双乳化液滴滴传感器,用于测量活组织中的透压. 这些传感器揭示了斑马鱼胚胎内平衡的细胞内和细胞外压力,有助于理解细胞和组织机制.

科学领域:

细胞力学细胞力学
发育生物学是发展生物学.
生物物理学的生物物理.

背景情况:

透压显著影响细胞和组织特征.
在复杂的生物系统中,在现场和体内测量透压是具有挑战性的.

研究的目的:

引入和验证用于局部化透压测量的双乳液滴滴传感器.
研究3D多细胞系统中的透压力动态,包括发育中的胚胎.

主要方法:

开发和校准双乳液滴滴传感器.
在斑马鱼胚胎胚泡和间隙液体中测量了透压的现场测量.
监测滴滴大小的变化以推断透压力.

主要成果:

在3D多细胞系统中成功地进行了透压的局部测量.
在斑马鱼胚胎中揭示了细胞内和介质透压 (~0.7 MPa) 之间的平衡.
确定了胚胎内部和外部之间的显著透压力不平衡.

结论:

双乳液滴滴传感器为in situ奥斯莫斯压力量化提供了一个新的工具.
这些发现有助于进一步了解透压在胚胎发育和组织平衡中的作用.
能够在未来对有机体和其它复杂生物环境中的透压进行研究.

更多相关视频

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Published on: February 11, 2020

相关概念视频

Osmosis and Osmotic Pressure of Solutions

Osmosis and Osmotic Pressure of Solutions

A number of natural and synthetic materials exhibit selective permeation, meaning that only molecules or ions of a certain size, shape, polarity, charge, and so forth, are capable of passing through (permeating) the material. Biological cell membranes provide elegant examples of selective permeation in nature, while dialysis tubing used to remove metabolic wastes from blood is a more simplistic technological example. Regardless of how they may be fabricated, these materials are generally...