在哺乳动物细胞中设计一个可扩展和直角的平台,用于合成通信
Anna-Maria Makri Pistikou1,2,3,4, Glenn A O Cremers1,2,3, Bryan L Nathalia1,2,3,4
1Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Nature communications
|November 3, 2023
概括
科学家们设计了一个合成哺乳动物通信系统,使用可编程受体和卷轴. 这个平台可以实现正交的细胞信号和逻辑操作,用于先进的细胞疗法.
科学领域:
- 合成生物学 合成生物学
- 细胞工程 细胞工程
- 生物技术是生物技术.
背景情况:
- 细胞通信电路是生物系统的基础.
- 工程合成通信平台可以推进细胞疗法.
- 现有的系统缺乏正交性和可扩展性.
研究的目的:
- 为哺乳动物细胞开发一个正交和可扩展的合成通信平台.
- 为治疗应用设计设计细胞联盟.
- 为了证明布尔逻辑运算和治疗性蛋白质表达.
主要方法:
- 使用可编程合成受体.
- 采用可调节,扩散的卷轴-卷轴化物用于选择性结合.
- 实施细胞间通信策略.
- 为 AND 门逻辑构建了一个三细胞群体系统.
主要成果:
- 在受体水平上证明了正交受体激活和布尔逻辑.
- 通过合成受体建立了细胞间的通信,并分泌了卷状线圈.
- 在三细胞系统中成功实现了AND门逻辑.
- 展示了CC-GEMS受体依赖的治疗性蛋白质表达.
结论:
- 开发的平台是模块化和可扩展的,用于工程复杂的细胞联盟.
- 这一框架在先进的细胞治疗和诊断中具有潜在的应用.
- 提供了合成细胞-细胞通信系统的基本原则.
相关概念视频
01:19Contact-dependent Signaling
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
01:21Cell-surface Signaling
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.