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IL-6在人类的ACL残留物中诱导皮奥斯的产生:一种可能导致创伤后关节炎的机制

Tzu-Hao Tseng^1,2, Chien-Lin Chen¹, Chung-Hsun Chang²

¹Department of Biomedical Engineering, College of Medicine, National Taiwan University, No.1 Jen Ai Road Section 1, Taipei City, 10002, Taiwan.

Journal of orthopaedic surgery and research

|November 3, 2023

概括

洲际蛋白-6 (IL-6) 刺激前十字带 (ACL) 遗留物产生素 (POSTN),这是由PI3K/Akt路径介导的过程. 这种相互作用可能会导致创伤后关节炎 (PTOA) 的发展.

科学领域:

生物医学研究的研究.
整形外科整形外科整形外科
分子生物学分子生物学

背景情况:

在前十字带 (ACL) 损伤后观察到高水平的质素 (POSTN) 和介质素-6 (IL-6).
怀疑ACL残留物是POSTN的主要来源,但IL-6在诱导POSTN产生中的作用尚不清楚.

研究的目的:

研究IL-6对ACL纤维细胞中POSTN产生的影响.
阐明 ACL 损伤和重建后创伤后关节炎 (PTOA) 的潜在影响和潜在的分子机制.

主要方法:

分析了27名接受ACL重建的患者的ACL残留物.
量化实时PCR和西班牙斑点被用来评估POSTN基因和蛋白质表达在用IL-6治疗的ACL纤维细胞中.
用PI3K/Akt,Ras/MAPK和JAK/STAT通路的抑制剂来识别涉及的信号通路.
进行了与ACL纤维细胞和软骨细胞的共同培养实验.

主要成果:

IL-6显著增加了ACL纤维细胞中的POSTN基因和蛋白质表达,以时间和剂量依赖的方式.
确定PI3K/Akt通路是IL-6诱导的POSTN产生的一个关键媒介.
同时暴露于IL-6和ACL残留物中的淋巴细胞增加了MMP-13和ADAMTS-4的表达.

结论:

IL-6诱导ACL残留物中的POSTN产生,这一过程因PI3K/Akt路径抑制而显著减弱.
IL-6和ACL残留物的联合存在可能会加速创伤后关节炎的进展.

关键词:

在ACL中,ACL是ACL.在IL-6的研究中,IL-6被认为是IL-6的基因.骨关节炎是一种骨关节炎.皮洛斯的时间.重建重建的重建工作

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