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通过综合生物信息学分析识别潜在的铁灭关键基因和类风湿性关节炎中的免疫透

Yihua Fan¹, Yuan Li¹, Xiaoyan Fu¹

¹Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China.

|November 3, 2023

概括

这项研究确定了关键的铁亡相关基因 (FRG) 和类风湿性关节炎 (RA) 的途径. 突出显示了VEGFA,PTGS2和JUN作为关键的枢纽基因,可能通过FoxO信号通路和免疫细胞透来调节RA.

科学领域:

生物医学研究的研究.
生物信息学是一种生物信息学.
免疫学免疫学免疫学

背景情况:

铁死在类风湿性关节炎 (RA) 的发病过程中起着重要作用.
了解分子机制,包括基因表达和免疫细胞参与,对于RA研究至关重要.
生物信息学方法提供了强大的工具来分析复杂的生物数据集在疾病如RA.

研究的目的:

识别与类风湿性关节炎 (RA) 相关的与铁亡相关的基因 (FRG).
阐明关键的信号通路和免疫细胞透模式,涉及RA的发病.
通过分析基因相互作用和差异性表达来发现潜在的治疗点.

主要方法:

从FerrDb获得的FRG和从GEO获得的RA基因表达数据 (GSE55235).
在RA中确定了差异表达基因 (DEG),并将其与FRG交叉.
使用STRING和Cytoscape来识别枢纽基因 (VEGFA,PTGS2,JUN) 构建的蛋白质蛋白相互作用 (PPI) 网络.
进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
分析了免疫细胞透模式.

主要成果:

在RA中鉴定出34个不同表达的与铁亡相关的基因 (34个FRG-DEG),其中7个是上调的,27个是下调的.
发现了三个关键的枢纽基因:VEGFA,PTGS2和JUN.
丰富分析显示了氧化应激反应,皮质类固醇反应,血红素结合和FoxO信号通路的参与.
在RA样本中观察到T细胞透的增加和M2巨细胞透的减少.

结论:

VEGFA,PTGS2和JUN被确定为关键的枢纽基因在与RA相关的铁亡中,主要与FoxO信号通路有关.
包括T细胞,巨细胞和血细胞在内的免疫细胞类型可能在RA相关的突炎炎中发挥调节作用.
这些发现为RA的分子机制提供了洞察力,并提出了潜在的干预目标.

关键词:

生物信息学分析铁化是铁化的一种.基因表达的总体.免疫系统的透.类风湿性关节炎类风湿性关节炎类风湿性关节炎

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