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瘤细胞SPTBN1通过抑制CXCL1表达来抑制巨细胞的M2极化

Huijie Wu¹, Nan Jiang¹, Jiajia Li²

¹Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, China.

Journal of cellular physiology

|November 3, 2023

概括

瘤细胞SPTBN1通过减少CXCL1.1来抑制巨细胞迁移和M2极化. 抑制癌细胞中的SPTBN1增强了TAMs,促进了瘤的生长.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

与瘤相关的巨细胞 (TAMs),特别是M2型,促进瘤进展并抑制抗瘤免疫力.
谱β非红细胞1 (SPTBN1) 在TAM极化中的作用在很大程度上是未知的.
以前的研究表明,SPTBN1可能会影响巨细胞的透.

研究的目的:

研究瘤细胞SPTBN1对肝瘤和乳腺癌中TAM极化和迁移的影响和机制.
为了确定SPTBN1是否影响M2极化和巨细胞迁移.
阐明涉及SPTBN1,CXCL1和p65.5的分子途径.

主要方法:

对瘤免疫数据库进行分析,以确定SPTBN1与免疫细胞的相关性.
反转录定量实时PCR (RT-qPCR) 和细胞迁移试验.
与癌细胞和M2巨细胞的共同培养实验,CXCL1中和ChIP-qPCR.

主要成果:

在瘤中发现SPTBN1和巨细胞/MDSC之间的负相关性.
在癌细胞中抑制SPTBN1增强了巨细胞迁移和M2极化,可通过CXCL1抗体逆转.
当与M2巨细胞共同培养时,癌细胞迁移和殖民地形成增加.
SPTBN1通过p65调节CXCL1,抑制其核定位.

结论:

瘤细胞SPTBN1通过减少CXCL1的表达和分泌来抑制TAM迁移和M2极化.
SPTBN1通过抑制p65核定位而起作用,从而调节CXCL1通路.
这些发现揭示了瘤SPTBN1影响瘤微环境的新型机制,并表明了潜在的治疗点.

关键词:

在CXCL1中,CXCL1是最重要的.SPTBN1 SPTBN1 是一个乳腺癌乳腺癌乳腺癌肝细胞癌是肝细胞癌.巨细胞表型开关是巨细胞表型开关.

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