在Candida albicans中,Nano-Ag可能对生物膜相关基因进行分子向
Mahbobeh Baghiat Esfahani1, Alireza Khodavandi2, Fahimeh Alizadeh3
1Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Applied biochemistry and biotechnology
|November 3, 2023
概括
银纳米粒子 (纳米Ag) 通过向关键的生物膜相关基因,有效地抑制Candida albicans生物膜. 这项研究证明了纳米-Ag.
科学领域:
- 微生物学 微生物学
- 纳米技术纳米技术
- 分子生物学分子生物学
背景情况:
- 在治疗 кандидоз感染方面,Candida albicans生物膜构成了重大挑战.
- 识别破坏生物膜形成的分子标对于有效的治疗策略至关重要.
研究的目的:
- 研究银纳米颗粒 (纳米-Ag) 作为潜在的分子剂,向Candida albicans中的生物膜相关基因.
- 评估生物合成的纳米-Ag. 的抗菌膜和抗浮游生物活性.
主要方法:
- 使用Bacillus licheniformis,Bacillus cereus和Fusarium oxysporum进行纳米Ag的生物合成.
- 使用光谱和显微镜对纳米Ag物理化学性质的表征.
- 通过MIC,MFC,XTT测定和晶体紫色染色来评估抗浮游生物和抗菌膜活性.
- 在纳米Ag处理的C. albicans.中对生物膜相关基因表达 (BCR1,ALS1,ALS3,HWP1,ECE1,TUP1,NRG1,TOR1) 的分析.
主要成果:
- 生物合成的纳米-Ag表现出显著的抗浮游生物和抗菌膜活动.
- 纳米Ag治疗导致关键生物膜形成基因 (BCR1,ALS1,ALS3,HWP1,ECE1) 的下调.
- 参与生物膜调节的负调节基因 (TUP1,NRG1,TOR1) 通过纳米Ag处理被上调调.
结论:
- 银纳米粒子通过调节特定毒性基因的表达,有效地抑制Candida albicans生物膜的形成.
- 用纳米Ag准生物膜相关基因代表了对抗 кандидоз感染的有前途的治疗策略.
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