隔离,功能评估,以及优化益生菌Bacillus coagulans的发酵过程
Li Xu1, Zhi Chun Zhan2,3, ShiShen Du1
1State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, P.R. China.
PloS one
|November 3, 2023
概括
一种新的Bacillus coagulans菌株,X26,显示出优越的益生菌特性,并显著改善了动物的体重. 优化的发酵实现了高产量,证明了其具有成本效益生产的工业潜力.
科学领域:
- 微生物学 微生物学
- 工业生物技术 工业生物技术
- 动物营养 动物营养
背景情况:
- 凝血杆菌是一种广泛用于各种行业的益生菌.
- 持续需要新型益生菌菌株,提高有效性和改进工业发酵过程.
研究的目的:
- 从土壤中分离和表征一种新的Bacillus coagulans菌株 (X26).
- 为了优化发酵条件,最大限度地提高B. coagulans的产量和子率X26.6.
- 评估B.coagulans X26作为动物补充剂的潜力.
主要方法:
- 从土壤样本中分离和鉴定B.凝血素X26.
- 对X26的酶,酸和生物质产量的比较分析与其他益生菌和抗生素相比.
- 使用响应表面方法来优化发酵参数 (麦芽糖,酵母提取物,K2HPO4).
- 发酵扩大规模的研究是在瓶式和工业发酵器 (30-L和10-T) 中进行的.
主要成果:
- 新的B.coagulans X26菌株显示出优异的酶,酸和生物质产量.
- 在动物试验中,X26显著改善了老鼠的体重.
- 优化发酵条件 (16.5g/L麦芽糖,25.00g/L酵母提取物,3.5g/LK2HPO4) 预测并实现了5.1 × 10^9 CFU/mL的产量.
- 在工业发酵器中进一步优化,在最佳温度 (45-55°C) 下,产量高达8.7 × 10^9 CFU/mL,子率高 (>96%).
结论:
- 细菌凝血菌X26是一种有前途的益生菌剂,具有重要的工业潜力.
- 优化的发酵过程允许成本高效,高产量的B.coagulans X26.26.的生产.
- 作为动物补充剂的X26的证明有效性需要进一步开发.
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