在哺乳动物细胞系中使用lentiviral系统的基编辑器对配列gRNA查的协议

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Updated: Jul 31, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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Nithin Sam Ravi1, Anila George1, Kumarasamypet M Mohankumar1

  • 1Centre for Stem Cell Research (a Unit of inStem, Bengaluru), Christian Medical College Campus, Bagayam, Vellore, Tamil Nadu 632002, India; Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695 011, India.

STAR protocols
|November 3, 2023
PubMed
概括

这项研究介绍了一种基于CRISPR的基因编辑协议,用于精确的基因组工程. 该方法促进了阵列突变发生,以确定调节元素,帮助遗传研究和目标发现.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?细胞培养 细胞培养遗传学 遗传学 是一个分子生物学分子生物学

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...