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Updated: Jul 11, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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空间蛋白质学的常规工作流程在微型素固定的抛嵌入组织上

Hao Chen1,2,3, Yuefei Zhang1,2,3, Haichao Zhou1,2,3

  • 1College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.

Analytical chemistry
|November 3, 2023
PubMed
概括

研究人员开发了一种常规的空间蛋白质组学工作流程,用于甲固定嵌入 (FFPE) 组织. 这种方法可以从小组织区域进行高分辨率的蛋白质识别,从而创建详细的空间蛋白质原子地图.

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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