使用形态学,HPLC,HPTLC和DNA条形码方法识别沙夫兰市场样本中的改
Varadharajan Bhooma1, Sophie Lorraine Vassou1, Ilango Kaliappan2
1Center for DNA Barcoding, Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, , SRM Institute of Science and Technology, SRM Nagar, Kattankulathur 603203, Kanchipuram, Chennai, TN, India.
Genome
|November 3, 2023
概括
沙夫兰经常被改. 通过DNA条形码,有效地识别了43%的造样品,包括未申报的植物物种和合成化合物,有助于质量控制.
科学领域:
- 食品科学 食品科学 食品科学
- 植物学 植物学
- 分析化学 分析化学
背景情况:
- 沙法兰 (Crocus sativus L. stigma) 是一种高价值的香料,由于其成本和供应有限,容易受到改.
- 伪造损害了沙夫兰的质量,安全性和经济完整性,需要可靠的检测方法.
研究的目的:
- 评估和比较形态学,高性能液态染色学 (HPLC),高性能薄层染色学 (HPTLC) 和DNA条形码在检测香伪造中的有效性.
- 为了识别用于商业沙夫兰样品中使用的伪造植物物种和填充剂.
主要方法:
- 沙夫兰污名的形态分析.
- 使用HPLC和HPTLC进行化学分析,以检测特定化合物和自我改.
- 用DNA条形码进行物种识别的分子分析.
主要成果:
- 在104个 (43%) 的沙夫兰样本中,有45个被发现是造的.
- 通过DNA条形码识别出最多的伪造样本 (44),检测出未申报的植物部分和20种不同的伪造物种.
- 在HPLC和HPTLC中,鉴定出了合成的沙夫兰和自我改 (沙夫兰植物部分).
结论:
- DNA条形码是检测广泛的香杂物,包括植物和合成的高级方法.
- 已识别的造物种对人类健康构成潜在风险,并强调需要严格的市场监督.
- 结合化学和分子方法的综合分析方法对于全面的沙夫兰质量控制至关重要.
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