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Updated: Jul 11, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

通过控制地释放Cas9核糖蛋白在植物细胞质中,使用聚合物修饰的微针阵列进行高效的基因组编辑

Anchu Viswan¹, Chiaki Yoshikawa², Ayana Yamagishi¹

¹Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.

Biochemical and biophysical research communications

|November 3, 2023

概括

研究人员使用一种新的聚合物修饰微针阵列 (MNA) 改进了将基因组编辑蛋白直接输送到植物中的方法. 这提高了无DNA作物编辑效率,绕过了传统遗传修饰方法的需要.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
生物工程是生物工程.

背景情况:

基因组编辑蛋白的直接输送为作物编辑提供了无DNA的方法,避免了向量DNA的整合.
之前的工作建立了微针阵列 (MNA) 介导的Cas9核糖蛋白 (RNP) 输送到植物组织.

研究的目的:

提高蛋白质传递效率,改善植物基因组编辑结果.
开发一种新的MNA修饰,以改善蛋白质固定和释放.

主要方法:

用生物活性聚合物 (DMA/HPA/NHS) 修改MNA以增强蛋白质结合.
在模仿植物细胞醇的环境中测试蛋白质释放.
在Arabidopsis thaliana中使用修饰和未修饰的MNA评估Cas9 RNP传递效率.

主要成果:

聚合物修饰促进了Cas9 RNPs和MNA之间更强的结合.
修改后的MNA在模拟的细胞环境中表现出更好的蛋白质释放.
在Arabidopsis thaliana的叶子中,使用聚合物修饰的MNA与未修饰的MNA相比,Cas9 RNP传递效率从4/17增加到9/17.

结论:

生物活性聚合物修饰MNA显著提高了基因组编辑蛋白直接输送到植物组织.
这种改进的传递方法有望开发更有效的无DNA植物基因组编辑技术.
进一步优化聚合物修饰条件可能会导致植物生物技术的更大进步.

关键词:

阿拉比多普西斯的叶子生物活性聚合物聚合物这就是Cas9的情况.基因组编辑基因组编辑微针阵列是一个微针阵列.是一种,是一种.

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