功能性微生物群的识别,这些微生物群是硫法酸污染的基质由Hermetia illucensens生物降解的基础
Jing Xia1, Chaorong Ge1, Huaiying Yao2
1Research Center for Environmental Ecology and Engineering, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, People's Republic of China.
Journal of hazardous materials
|November 3, 2023
概括
黑士兵幼虫 (BSFLs) 可以降解硫亚 (SDZ) 抗生素. 通过DNA稳定同位素探测,确定了参与这一关键环境修复过程的关键微生物属.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 环境中的抗生素残留物加快了抗生素耐药性.
- 在现场识别降解抗生素的微生物是具有挑战性的.
- 生物降解为消除抗生素提供了一个有希望的解决方案.
研究的目的:
- 在黑士兵幼虫 (BSFL) 的肠道中识别活性硫法迪亚 (SDZ) 降解微生物.
- 阐明BSFL肠道微生物群对SDZ的现场降解机制.
主要方法:
- 使用13C6标记的SDZ.Z.进行DNA稳定同位素探测 (DNA-SIP).
- 随着时间的推移,SDZ降解效率的量化.
- 微生物群落的同时发生网络分析.
- 代谢途径分析.
主要成果:
- 在6天内,BSFL在100毫克/公斤-1的剂量下实现了73.99%的SDZ降解.
- DNA-SIP确定了9个属,积极降解SDZ.
- 观察到特定的SDZ-降解一般性之间的正相关性.
- 在SDZ生物降解过程中,碳水化合物代谢,膜运输和翻译都受到上调.
结论:
- 在BSFL的肠道中,有一个独特的微生物群落,能够生物降解硫法.
- DNA-SIP有效地用于在现场识别降解抗生素的微生物.
- 了解这些微生物群落和机制有助于制定抗生素污染控制策略.
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