新的罗达胺基传感器用于高灵敏度光追踪Cys,同时对H2S进行色度检测
Yanyun Mao1, Qiangmin Yu1, Tianqing Ye2
1College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|November 3, 2023
概括
这项研究引入了一种基于罗达胺的新型传感器FR,用于选择性光检测氨酸 (Cys) 和色度检测硫化 (H2S). 这种双动感应器为监测这些关键生物分子提供了灵敏和可视的方法.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 含有硫的化合物,如氨酸 (Cys),同氨酸 (Hcy),氨酸 (GSH) 和硫化 (H2S),在许多生理过程中至关重要.
- 使用光学传感器在单个分子水平上区分和检测这些生物醇是一个重大挑战.
研究的目的:
- 开发一级合成的基于罗达胺的传感器 (FR),能够同时检测Cys和H2S.
- 为了实现Cys和H2S的特定光和色度反应,分别.
主要方法:
- 基于罗达胺的传感器 (FR) 的单步合成,利用和基作为响应部位.
- 使用1H NMR和质谱法对传感器响应机制的表征.
- 对检测细胞内Cys变化的传感器性能进行评估.
主要成果:
- 传感器FR在565nm对Cys具有特定的光增强,具有高选择性和低检测极限 (49nM).
- 传感器在暴露于H2S时显示出明显的颜色变化,从无色到蓝紫,从而使可视色度检测.
- 罗达胺结构的封闭状态有助于低背景光信号,增强Cys检测灵敏度.
结论:
- 开发的传感器FR提供了一种敏感和选择性的方法,用于同时检测Cys和H2S.
- 传感器FR有效监测细胞内Cys水平的外源和内源变化.
- 这种传感器为在生物系统中检测生物醇提供了有价值的工具.
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