TAZ通过Pard3-p38 MAPK-TAZ信号轴刺激运动诱导的肌肉卫星细胞激活
Kyung Min Kim1, Gi Don Yoo1, Woong Heo1
1Division of Life Sciences, Korea University, Seoul, Korea.
Journal of cachexia, sarcopenia and muscle
|November 3, 2023
概括
具有PDZ结合动机 (TAZ) 的转录共激活剂通过激活肌肉卫星细胞对肌肉再生至关重要. 塔兹 (TAZ) 是一个名为塔兹 (TAZ) 的城市.
科学领域:
- 肌肉生物学 肌肉生物学
- 细胞再生 细胞再生
- 分子机制的分子机制
背景情况:
- 运动刺激肌肉卫星细胞进行再生.
- 驱动这一过程的精确分子机制尚不清楚.
- 这项研究研究了具有PDZ结合动机 (TAZ) 的转录共激活剂在肌肉卫星细胞激活中的作用.
研究的目的:
- 阐明TAZ在肌肉卫星细胞激活和再生中的作用.
- 研究涉及TAZ的信号通路,以应对运动和肌肉损伤.
- 探索TAZ激活对与年龄相关的肌肉损失的治疗潜力.
主要方法:
- 生成的肌肉卫星细胞特异性TAZ淘汰 (sKO) 鼠标.
- 利用了耐力训练和肌肉损伤模型.
- 通过组织学和分子技术分析卫星细胞数量,肌肉再生和纤维直径.
- 在体外和体内研究了p38 MAPK-TAZ信号轴.
主要成果:
- 卫星细胞中TAZ的减少显著减少了肌肉再生和卫星细胞数量.
- TAZ与Pax7相互作用,促进Myf5的表达并激活卫星细胞.
- 由Pard3诱导的p38 MAPK-TAZ信号轴对于肌肉再生至关重要.
- 运动训练增加了TAZ水平,促进了肌肉纤维缩,SKO小鼠的效果减弱.
结论:
- 在受伤和炼后的卫星细胞激活和肌肉再生中,TAZ起着至关重要的作用.
- p38 MAPK-TAZ通路是这些过程的关键调节者.
- 向TAZ激活是针对肉症和与年龄相关的肌肉衰退的潜在治疗策略.
相关概念视频
01:22PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
01:21Satellite Stem Cells and Muscular Dystrophy
2.0K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.0K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
01:07MAPK Signaling Cascades
5.6K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.6K
01:16TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
01:20The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K