诊断和开始的Staphylococcus pseudintermedius基因组透露了通过平行选择防御机制的利基适应
Sanjam S Sawhney1, Rhiannon C Vargas1, Meghan A Wallace2
1The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Nature communications
|November 4, 2023
概括
斯塔菲洛科克 (Staphylococcus pseudintermedius) 适应不同的环境,在诊断环境中表现出更强的耐药性,在家庭环境中表现出更强的防御机制. 这种细菌适应是由利基驱动的,而不仅仅是宿主物种.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 伪中间体葡萄球菌 (Staphylococcus pseudintermedius) 是狗中常见的细菌,但在人类中越来越多地发现.
- 目前尚不清楚S. pseudintermedius的种群是否因其环境或宿主物种而有所不同.
研究的目的:
- 为了研究来自不同来源的Staphylococcus pseudintermedius分离物之间的基因组差异.
- 为了确定或宿主物种是否驱动细菌群体差异化.
主要方法:
- 501个Staphylococcus pseudintermedius分离物的比较基因组学.
- 分离物来源于人类诊断,动物诊断,人类殖民,物殖民和家庭表面环境.
- 分析包括核心和辅助基因架构,编码和表型抵抗以及CRISPR防御系统.
主要成果:
- 从诊断环境中分离出来的Staphylococcus pseudintermedius菌种显示出更高的编码和表型耐药性.
- 来自殖民和表面环境的分离物共享了类似的CRISPR防御系统,这表明了常见的菌体暴露.
- 经过脱殖民化的家庭隔离物体表现出防御基因的并行进化和对某些抗生素的敏感性降低.
结论:
- 利基,而不是宿主物种,驱动Staphylococcus pseudintermedius种群差异化.
- 在S. pseudintermedius中,防御机制通过基因获取或突变以利基特定的方式得到加强.
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