相关实验视频

Updated: Jul 11, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

在多个转录基因队列中对替代拼接的全面分析揭示了前列腺癌的预后特征

Zhuofan Mou¹, Jack Spencer², John S McGrath^1,3

¹Clinical and Biomedical Sciences, Department of Clinical and Biomedical Sciences, University of Exeter Medical School, Faculty of Health and Life Sciences, University of Exeter, Barrack Road, Exeter, EX2 5DW, UK.

|November 4, 2023

概括

替代拼接变化是前列腺癌 (PCa) 进展的关键. 这项研究确定了新的拼接事件,并开发了一个准确预测PCa患者生化无复发生存率 (BCRFS) 的签名.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

替代拼接 (AS) 对于转录组多样性至关重要,也是癌症的标志,显著影响前列腺癌 (PCa) 的发展和进展.
对AS的失调越来越多地与PCa的发病和进展有关.
需要对AS概况进行全面的分析,以预测PCa的预后.

研究的目的:

在前列腺癌中进行替代拼接事件的综合分析.
确定与患者预后和生化无复发生存率 (BCRFS) 相关的新型AS事件.
根据AS事件开发和验证PCa的预后签名.

主要方法:

在三个独立的队列 (数组和RNA测序) 中对AS事件的差异表达分析.
确定与BCRFS显著相关的AS事件.
开发一个六个事件的预后签名和一个整合格里森分数和瘤阶段的诺莫图.

主要成果:

在ZWINT中确定了一种保留的内部事件.
发现了141个改变的AS事件,其中21个与BCRFS显著相关.
开发的AS签名在预测5年的BCRFS方面表现优于传统标记,并显示出强大的验证.

结论:

在PCa中发现了与BCRFS相关的新型拼接事件.
开发的预后签名和名谱改善了PCa临床进展的预测.
这项研究为完善前列腺癌管理中的临床决策提供了增强的工具.

关键词:

另一个替代拼接.一个微阵列的微阵列.预测预后预测预测前列腺癌是什么意思前列腺癌是什么意思在RNA-seqqq.

更多相关视频

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

相关概念视频

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...