相关实验视频

Updated: Jul 11, 2025

Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model

Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model

Published on: April 18, 2025

在人类糖尿病心肌病中,非编码RNA介导的内皮细胞转变为介质细胞,通过DNA甲基化进行潜在调节

Eric Wang¹, Shali Chen¹, Honglin Wang¹

¹Department of Pathology and Laboratory Medicine, Western University, Dental Science Building Room 4033, 1151 Richmond St, London, ON, N6A 3K7, Canada.

Cardiovascular diabetology

|November 4, 2023

概括

内皮转介质转变 (EndMT) 在人类糖尿病心肌病心脏中发生,与非编码RNA (ncRNA) 的变化相吻合. DNA甲基化与ncRNA相互作用,影响糖尿病心脏中的EndMT.

科学领域:

心血管科学心血管科学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

糖尿病心肌病 (DCM) 是一种与心血管疾病相关的严重糖尿病并发症.
内皮质功能障碍,特别是内皮质到介质酶过渡 (EndMT),是DCM病原体的核心.
非编码RNAs (ncRNAs) 的表观遗传调节与EndMT有关,但人类研究是有限的.

研究的目的:

在人类糖尿病心脏中确认EndMT,反映动物和细胞研究的发现.
调查ncRNAs在人类DCM中的EndMT中的作用.
探索与DCM相关的EndMT中DNA甲基化和ncRNA之间的潜在相互作用.

主要方法:

来自尸检患者的人类心脏组织使用组织学和免疫光学进行了分析.
定量逆转录-聚合酶连锁反应 (RT-qPCR) 评估了ncRNA表达.
对组织样本进行了DNA甲基化分析.

主要成果:

糖尿病患者的心脏表现出心脏纤维化增加和EndMT的证据.
通过RT-qPCR确认了人类糖尿病心脏的EndMT,并确定了关键ncRNAs的相应变化.
甲基化分析显示,DNA促进物甲基化调节了一些EndMT相关的ncRNAs.

结论:

EndMT是人类DCM的一个相关的病理过程,与改变的ncRNA表达相关.
DNA甲基化和特定的ncRNA之间的相互作用有助于DCM中的EndMT调节.
这些发现凸显了DCM和EndMT患者背后的复杂表观遗传机制.

关键词:

糖尿病心肌病糖尿病心肌病内皮细胞到介质细胞的过渡.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.长的非编码RNA是什么?促进剂甲基化促进剂甲基化这是一个微型RNA.

更多相关视频

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Published on: February 26, 2021

相关概念视频

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Coronary Artery Disease I: Introduction

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...