SCR106拼接因子通过维持大米中的RNA拼接来调节无生物应激反应
Abdulrahman Alhabsi1, Haroon Butt1, Gwendolyn K Kirschner2
1Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
Journal of experimental botany
|November 4, 2023
概括
一种新型的米拼接因子,OsSCR106,对于植物非生物应激耐受性至关重要. 这种富含氨酸/氨酸的蛋白质调节mRNA前剪接,确保适当的基因表达和在压力下的发育.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因调节 基因调节
- 无生物应激反应的应激反应
背景情况:
- 植物利用复杂的分子机制来进行生长,发育和应激适应.
- 基因表达,包括转录和剪接,控制细胞反应.
- 富含氨酸/氨酸 (SR) 的蛋白质是前mRNA剪接的关键调节者,影响基因表达的多样性.
研究的目的:
- 识别和描述大米中的新型拼接因子.
- 研究OSSCR106在mRNA前拼接和非生物应激反应中的作用.
- 阐明OSSCR106功能所需的分子机制.
主要方法:
- 在大米中发现了一种新的SR蛋白相关因子OSSCR106.
- 对OsSCR106在核内定位的分析 (核斑).
- 在各种压力条件下 (盐,ABA,低温) 对功能丧失的scr106突变体的表型分析.
- 补充测试以确认OSSCR106的功能.
- 全球基因表达和全基因组拼接分析.
主要成果:
- 含有RNA识别基因 (RRM) 和SR二的OSSCR106属于SC亚家族.
- 这种scr106突变体对盐,酸和低温压力过敏,以及发育缺陷 (较短的芽/根).
- 通过补充验证了OSSCR106在耐压力中的功能.
- OsSCR106调节了目标基因拼接,可能是通过替代的3'-拼接位控制,在盐应力下影响多个拼接异型.
结论:
- OsSCR106是大米中重要的拼接因子,对于准确的mRNA前拼接至关重要.
- OsSCR106在调解植物对非生物应激反应方面发挥着至关重要的作用.
- 这项研究强调了OsSCR106作为通过拼接调节来调节应力适应的关键调节器.
相关概念视频
02:18Alternative RNA Splicing
21.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.2K
02:41Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
01:56Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
01:29Translational Regulation
25
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
25
01:36Pre-mRNA Processing: RNA Splicing
5.3K
5.3K
02:41Chromatin Structure and RNA Splicing
2.7K
2.7K