相关实验视频

Updated: Jul 11, 2025

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

Published on: January 25, 2011

枢纽基因和与牙无牙生物膜相关的途径:临床元转录学研究

Laís Daniela Ev¹, Joice de Faria Poloni², Nailê Damé-Teixeira³

¹Department of Preventive and Social Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.

Clinical oral investigations

|November 4, 2023

概括

没有的生物膜具有独特的功能特征,具有独特的基因表达模式. 像HSPA1s,Epr和SLC1A这样的基因可以作为维持口腔健康和预防的生物标志物.

科学领域:

微生物组研究的研究.
口腔微生物学口腔微生物学
大基因组学和转录组学.

背景情况:

牙损是一种由口腔微生物群影响的多因素疾病.
对于预防和治疗策略来说,了解口腔生物膜在病状态中的功能差异至关重要.

研究的目的:

在没有 (CF),停止 (CI) 和活性 (CA) 个体中研究supragingival生物膜的微生物功能概况.
为了确定与每个病状况相关的特定基因和途径.

主要方法:

从14个人 (6CF,3CI,5CA) 的supragingival生物膜的metatranscriptomic分析.
RNA提取,cDNA合成,以及高通量测序 (Illumina HiSeq3000).
使用SortMeRNA,SqueezeMeta管道,DESeq2进行差异基因表达和WGCNA进行网络分析的生物信息分析.

主要成果:

鉴定了5303个基因;协同表达网络分析揭示了与状况相关的模块.
无虫 (CF) 生物膜与特定的基因模块显示出强烈的正相关性 (r=0.9).
关键基因 (HSPA1s,Epr,SLC1A) 和途径 (例如,碳水化合物代谢,鞭毛组合) 与CF状况显著相关.

结论:

一个独特的功能特征标志着无虫的生物膜,与同时发生的基因使同时途径活动.
建议HSPA1s,Epr和SLC1A基因作为潜在的生物标志物用于无虫的生物膜.
识别这些生物标志物可以提高虫病预防和控制策略的知识.

关键词:

生物膜是一种生物膜.牙腐烂是一种牙腐烂.基因表达基因表达基因表达微生物群中的微生物群在RNA-Seqq.转录组转录组就是一个转录组.

更多相关视频

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

相关概念视频

Biofilms

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...