在低氧条件下改善微生物生物生产
Shawn Kulakowski1, Deepanwita Banerjee1, Corinne D Scown2
1Joint BioEnergy Institute, Emeryville, CA 94608, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Current opinion in biotechnology
|November 4, 2023
概括
微生物生物生产可以通过工程微生物菌株在低氧条件下得到改善. 这种方法克服了无氧和有氧过程的局限性,为工业扩展铺平了道路.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 由于氧气转移成本,工业生物生产往往有利于无氧条件.
- 无氧生物转化面临限制,包括限制基质使用,降低产量和减少产品多样性.
- 有氧过程提供了优势,但并不总是可行的或最佳的.
研究的目的:
- 开发能够在低氧条件下高效生物生产的微生物菌株.
- 克服完全无氧和有氧生物过程固有的局限性.
- 为工业应用建立一个可扩展的生物生产平台.
主要方法:
- 利用氧化还原辅助因子工程来优化微生物代谢.
- 采用基因组规模的代谢建模用于途径分析和预测.
- 应用功能基因组学以识别和验证关键的遗传修饰.
- 专注于微生物宿主工程,以提高低氧性能.
主要成果:
- 在氧气水平降低的情况下,已证明改善了生物生产.
- 与传统无氧方法相比,提高了基质利用率和产品产量.
- 扩大了在低氧生物工艺中可实现的潜在产品多样性.
- 开发了一个可行的策略,用于扩大工程生物生产系统的规模.
结论:
- 微生物菌株工程为低氧生物生产提供了强大的解决方案.
- 整合氧化还原因子工程,代谢建模和功能基因组学是成功的关键.
- 这项工作为可持续的工业生物生产提供了一个可扩展的途径.
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