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相关概念视频

RNA Structure01:23

RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Nucleic acids02:43

Nucleic acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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相关实验视频

Updated: Jul 11, 2025

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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聚甲基RNA的溶液结构

Cristian A Escobar1, Riley J Petersen1, Marco Tonelli2

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.

Journal of molecular biology
|November 4, 2023
PubMed
概括
此摘要是机器生成的。

研究人员确定了自由多样性UG (pUG) RNA的溶液结构,揭示了一个紧的,左手四重复结构. 这种"pUG折叠"与与连接体结合的结构一致,表明它是连接体.

关键词:
这就是NMR的NMR.这是一个四重复式住宅.这是一个RNARNARNARNARNA.萨克斯 (SAXS) 的时间聚聚 (UG) 是一种

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 在RNA生物学,RNA生物学.

背景情况:

  • 多格 (pUG) RNAs是丰富的真核细胞序列,参与基因沉默.
  • 之前的研究确定了pUGRNA与N-甲基介质氨酸IX (NMM) 结合的晶体结构.
  • 自由的pUGRNA的结构仍然是未知的.

研究的目的:

  • 为了确定自由多样性 (UG) RNA的溶液结构.
  • 调查pUG折叠的结构基础及其稳定性.

主要方法:

  • 核磁共振 (NMR) 光谱学是指核磁共振 (NMR) 的光谱学.
  • 微小和广角X射线散射 (SAXS/WAXS) 技术
  • 特定站点的脱氧酶修改,以帮助NMR信号分配.

主要成果:

  • 自由 (GU) 12RNA的溶液结构使用NMR-SAXS-WAXS.S.来确定.
  • 一个紧的,左手四重体结构与Z形脊柱,称为"pUG折叠",被确定.
  • 确定的结构与NMM结合的pUGRNA的晶体结构一致,表明折叠不受连接物结合的改变.

结论:

  • 格折叠是自由格RNA的稳定结构图案.
  • 溶液结构提供了结晶学无法解决的形状细节.
  • 这些发现解释了pUG折叠如何在较长的RNA分子中形成.