沙门氏菌 Typhimurium 效应器 SpvB 通过准克拉特林和 AP-1 来颠覆宿主膜贩运
Yi Yuan1, Xinghao Wang2, Jie Jin1
1Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China; NHC Key Laboratory of Medical Immunology, Peking University, Beijing, China.
Molecular & cellular proteomics : MCP
|November 4, 2023
概括
沙门氏菌SpvB蛋白向克拉和适应蛋白1,破坏宿主细胞过程. 抑制适应蛋白1可以提高宿主细胞内的沙门氏菌存活率.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 沙门氏菌 (Salmonella enterica) 引起胃肠道和全身疾病.
- SpvB是一种沙门氏菌毒性因子,通过其C端ADP-ribosyltransferase域修改宿主活性.
- 斯宾塞堡的N端功能和额外的目标在很大程度上是未知的.
研究的目的:
- 为了研究SpvB N-终端域的功能.
- 为了确定SpvB.B.针对的其他宿主因素.
- 了解SpvB与宿主相互作用对沙门氏菌病原性的影响.
主要方法:
- 共同免疫沉测试以确定SpvB相互作用伙伴.
- 西方涂抹用于检测蛋白质修饰.
- 通过RNA干扰 (RNAi) 来降低适应蛋白1 (AP-1) 的表达.
- 细胞培养模型用于研究沙门氏菌感染动态.
主要成果:
- SpvB通过其N端域直接与克拉和适应蛋白1 (AP-1) 相互作用.
- 与克拉林/AP-1的SpvB结合会破坏克拉林介导的细胞内和蛋白质分泌.
- 抑制AP-1显著提高了细胞内沙门氏菌的存活率和增殖率.
结论:
- SpvB的N端准了克拉和AP-1,干扰了重要的宿主细胞贩运通路.
- 通过SpvB干扰克拉特林介导的内细胞和AP-1功能,有助于沙门氏菌的发病.
- 准AP-1是控制沙门氏菌感染的潜在策略.
相关概念视频
Pinching-off of Coated Vesicles
3.2K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.2K
Clathrin Coated Vesicles
7.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.0K
Membrane Asymmetry Regulating Transporters
4.5K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.5K
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
Rab Proteins
3.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.9K
Overview of Secretory Vesicles
8.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.6K


