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来自日本常见鱼Todarodes pacificus的一种新发现的酶具有生物合成d-aspartate的能力

Hiroki Koyama¹, Yui Takahashi¹, San Matori²

¹Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, Japan.

Archives of biochemistry and biophysics

|November 4, 2023

概括

科学家在鱼中发现了一种新的D-阿斯巴酸种族酶 (AspRase),挑战了关于酶结构的先前假设. 这种独特的aspRase与阿斯巴酸氨基转移酶相关,具有双重的酶活性.

科学领域:

生物化学生物化学
酶学是一种酶学.
进化生物学进化生物学

背景情况:

氨基酸以L和D异构体的形式存在,L-氨基酸在体内占主导地位.
D-阿斯巴酸 (D-Asp) 起着至关重要的生理作用,由阿斯巴酸种族酶 (AspRase) 从L-Asp合成.
D-Asp在头足动物的神经系统中积累,但AspRase基因在这些物种中仍然未被识别.

研究的目的:

在头足动物中识别编码AspRase的基因.
描述小说"AspRase"的生物化学特性和遗传学关系.
了解D-Asp生物合成途径及其演变.

主要方法:

从 *Todarodes pacificus* (日本常见鱼) 的光学质中进行基因鉴定.
生物化学测试以确定酶活性,最佳pH值和温度.
遗传学分析以确定进化关系.

主要成果:

一个编码AspRase的新型基因在日本常见鱼中成功被识别出来.
鱼AspRase表现出独特的结构和植物遗传特征,与已知的AspRase显著不同.
该酶具有AspRase和阿斯巴酸氨基转移酶 (AST) 活动,在pH 7.0和20°C时具有最佳活性.
AspRase的活性是由2-oxoacids增强的.

结论:

在鱼中发现一种独特的,与AST相关的aspRase挑战了动物aspRases的保存结构假设.
这一发现表明,哺乳动物的aspRases也可能与AST有关.
这项研究为D-Asp生物合成途径的演变提供了关键的见解.

关键词:

亚斯巴酸氨基转移酶是一种亚斯巴酸氨基转移酶.亚斯巴酸盐的种族性质.人类遗传学分析通过RNA-Seq分析进行分析.d-阿斯巴酸盐的使用方法

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