来自海洋生物质衍生的银河体中的l-histidine的生物合成在代谢工程Corynebacterium glutamicum中
Minhye Kim1, Jun Won Oh1, Da Woon Jeong2
1Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Bioresource technology
|November 4, 2023
概括
研究人员开发了一种可持续的方法来生产l-histidine,使用来自红藻的D-galactose. 这涉及修改Corynebacterium glutamicum以有效利用银河糖,显著提高工业应用的l-histidine合成.
科学领域:
- 生物技术是生物技术.
- 海洋生物质的利用海洋生物质的利用
- 代谢工程是代谢工程.
背景情况:
- 食品和药品中对l-histidine的需求不断增加,需要可持续的生产方法.
- 海洋生物质,特别是像 κ-carrageenan 这样的红藻,提供了潜在的可再生碳来源.
- 有效地将海产糖转化为有价值的化合物是一个持续的挑战.
研究的目的:
- 开发一种环保的l-histidine合成工艺,使用来自海洋生物质的D-galactose.
- 为了改造Corynebacterium glutamicum以提高D-银糖的利用率和l-histidine的生产.
- 评估使用水解红藻作为可持续原料的可行性.
主要方法:
- 使用 κ-carrageenase (CgkA) 和 iduronate-2-sulfatase (IdsA3) 来化 κ-carrageenan,以获得 D-银糖.
- 通过修改前体路径来增强l-histidine合成,对Corynebacterium glutamicum的代谢工程.
- 在工程菌株中引入D-银糖利用系统,创建菌株TDPH6G2.2.
主要成果:
- 与母菌株TDP相比,TDPH6菌株的l-histidine产量增加了2.15倍.
- 工程菌株TDPH6G2在发酵过程中有效地消耗了100%的D-银糖.
- 菌株TDPH6G2成功合成了0.395g/L的l-histidine,证明了有效的银河糖同化.
结论:
- 可持续的l-histidine生产可以使用来自海洋生物质的D-银糖来实现.
- 具有银河糖利用系统的工程Corynebacterium glutamicum是增强l-histidine合成的可行策略.
- 这项研究为在工业生物生产中利用海洋资源提供了基础.
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