自体抗体子类的主导性不是由异常的类切换或B细胞发育受损所驱动的
Laurent M Paardekooper1, Yvonne E Fillié-Grijpma1, Alita J van der Sluijs-Gelling2
1Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands.
Clinical immunology (Orlando, Fla.)
|November 4, 2023
概括
在IgG4相关的自身免疫性疾病中,B细胞的发育正常. IgG4自身抗体的优势很可能是由特定抗原驱动的,而不是异常的B细胞成熟.
科学领域:
- 免疫学 免疫学 免疫学
- 这是一种自身免疫力.
- B细胞生物学B细胞生物学
背景情况:
- 一种自身免疫性疾病的子集具有主要的致病性免疫球蛋白G4 (IgG4) 自体抗体,称为IgG4-AIDs.
- 导致IgG4亚类在这些疾病中占主导地位的潜在机制在很大程度上是未知的.
- 这项研究调查了潜在的原因,包括B细胞成熟和类切换异常.
研究的目的:
- 调查B细胞成熟失调或异常类别切换是否导致IgG4-生成B细胞和IgG4-AIDs中的血细胞过度代表.
- 为了比较患有IgG4-AIDs的患者与患有IgG1-3-AIDs和健康个体的B细胞区.
主要方法:
- 流细胞计用于分析四种不同的IgG4-AID,两种IgG1-3-AID和健康捐赠者的B细胞区.
- 分析的重点是所有成熟阶段的相对子集丰度和特定细胞群的量化.
主要成果:
- B细胞成熟阶段显示IgG4-AIDs的正常亚群丰度,不成熟和天真的CD5+细胞可能与治疗相关的减少.
- 产生IgG4的B细胞和血细胞数量与对照细胞数量相似.
- 在缺乏自身活性的IgG4-AID患者中,观察到循环CD20-CD138+细胞的显著,独立于子类的8倍增加.
结论:
- 这些发现表明,异常的B细胞发育不是IgG4亚类在这些自身免疫性疾病中占主导地位的主要驱动因素.
- 这些结果支持针对特定自身抗体子类的抗原驱动机制.
- 尽管临床表现不同,但IgG4-AIDs具有共同的潜在免疫特征.
相关概念视频
01:25Antibody Structure and Classes
926
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
926
01:24B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
01:10Antibody Structure
60.2K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
60.2K
01:20Special Features of Adaptive Immunity
836
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
836
01:28Diversity of Antigen Receptors
599
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
599
01:31Hybridoma Technology
14.8K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
14.8K