相关实验视频

Updated: Jul 11, 2025

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

基础分辨率定量DAMM-seq用于映射tRNA和线粒体多基斯特龙RNA中的RNA甲基化

Li-Sheng Zhang¹, Cheng-Wei Ju², Bochen Jiang³

¹Division of Life Science, The Hong Kong University of Science and Technology (HKUST), Hong Kong SAR, P.R. China; Department of Chemistry, The Hong Kong University of Science and Technology (HKUST), Hong Kong SAR, P.R. China; Department of Chemistry, The University of Chicago, Chicago, IL, United States; Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, United States.

Methods in enzymology

|November 4, 2023

概括

人类ALKBH7是一种新型的线粒体RNA脱甲基酶,可以从前tRNA中去除特定的甲基化,从而影响线粒体RNA的处理和结构.

科学领域:

线粒体生物学线粒体生物学
基因组RNA的修饰是RNA的修饰.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

包括FTO和ALKBH5在内的AlkB家族蛋白质是已建立的RNA m6A脱甲基酶.
影响新陈代谢的线粒体蛋白ALKBH7的确切功能和机制在很大程度上是未知的.

研究的目的:

阐明人类ALKBH7.7的生物功能和机制.
为了确定线粒体内ALKBH7的特定RNA基质和脱甲基化点.

主要方法:

开发脱甲基化辅助多重甲基化测序 (DAMM-seq),用于同时检测多个RNA甲基化 (m¹A, m³C, m¹G, m²2G).
应用DAMM-seq来分析稳定状态和新生RNA,重点关注线粒体多基斯特龙RNA.

主要成果:

ALKBH7被确定为一种脱甲基酶,向N²,N²-二甲基瓜诺辛 (m²₂G) 和N¹-甲基亚诺辛 (m¹A) 的修饰.
这些脱甲基化事件发生在线粒体多基斯特龙RNA内的特定tRNA前区域 (mt-Ile和mt-Leu1).
DAMM-seq揭示了ALKBH7在调节RNA处理和新生的线粒体RNA中局部结构变化的作用.

结论:

人类ALKBH7在线粒体中作为一种新型RNA脱甲基酶起作用.
ALKBH7特别针对线粒体前tRNA中的m²2G和m¹A,影响RNA处理.
这项研究描述了ALKBH7的基质特异性和机制,解决了RNA生物学中长期存在的问题.

关键词:

ALKBH7H7 在这里.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.线粒体中的线粒体.下一代测序是下一代测序.脱甲基化RNA脱甲基化

更多相关视频

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...