含有银的酸涂层在体外和体外对金黄色葡萄球菌的长期抗菌活性
Sakumo Kii1, Hiroshi Miyamoto2, Masaya Ueno1
1Department of Orthopaedic Surgery, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
概括
银酸 (Ag-HA) 涂层释放银离子超过六个月,有效抑制细菌生长和生物膜形成. 这些发现表明,Ag-HA涂层可以帮助预防晚发性骨科植入物感染.
科学领域:
- 生物材料科学 生物材料科学
- 整形外科手术 整形外科手术
- 传染性疾病 传染性疾病
背景情况:
- 银亚酸盐 (Ag-HA) 涂层在预防骨科植入物感染方面表现有前途.
- 对抗晚发性感染的Ag-HA涂层的疗效在很大程度上仍未得到研究.
研究的目的:
- 为了评估从Ag-HA涂层中长期的银离子 (Ag+) 化.
- 在体外和体内评估Ag-HA涂层的抗菌和抗生物膜特性.
主要方法:
- 用Ag-HA涂层的磁盘在胎儿牛血清中化长达6个月,通过ICP-MS测量Ag+化.
- 在体外研究中,用MRSA对Ag-HA和HA盘进行了注射,以量化细菌殖民地和生物膜形成.
- 在老鼠体内研究评估了MRSA或S. aureus Xen36接种后的细菌负荷和生物发光.
主要成果:
- 从Ag-HA涂层中观察到持续的Ag+化,持续超过6个月.
- 与体外HA涂层相比,Ag-HA涂层显著减少了细菌的数量和生物膜的形成.
- 在体内实验中,Ag-HA组的细菌负载和生物发光率较低.
结论:
- Ag-HA涂层提供长期的Ag+化,表现出强大的抗菌和抗生物膜活性,对S. aureus.
- Ag-HA涂层具有显著的潜力,可以缓解晚发性骨科植入物相关的感染.
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