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在猪中,AIRN lncRNA被印记并以父性表达

Jinsoo Ahn¹, In-Sul Hwang^2,3, Mi-Ryung Park²

¹Department of Animal Sciences, The Ohio State University, Columbus, OH 43210.

Journal of animal science

|November 5, 2023

概括

猪的基因组印记涉及Airn lncRNA,它是父表达的,并与母甲基化印记联系在一起. 这项研究确定了Airnn的身份.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.
发展生物学发展生物学

背景情况:

基因组印记对于哺乳动物发育至关重要,通常涉及长非编码RNA (lncRNAs).
这种Airn lncRNA调节了小鼠Igf2r基因的印记表达.
虽然DNA甲基化印记在猪中得到保存,但Airn lncRNA在猪中的作用仍然未被描述.

研究的目的:

为了研究猪Airn lncRNA的印记基表达.
确定和描述与Airn.相关的差异甲基化区域 (DMR).
分析猪中Airn和Igf2r的等位体表达.

主要方法:

部分遗传和对照猪胚胎的比较.
在特定区域分析DNA甲基化模式.
在不同组织和胚胎中对Airn和Igf2r基因表达的定量评估.

主要成果:

在猪胚胎中发现了一种母体甲基化DMR.
猪在对照胚胎中表现出明显更高的Airn lncRNA表达,表明父表达 (P < 0.05).
Airn lncRNA是单基表达的,主要在猪大脑中,而Igf2r显示双基表达.

结论:

这项研究证实了猪的Airn lncRNA的父表达,与母甲基化印记相关.
在猪大脑中,Airn lncRNA的表达得到了丰富,并且是单基的.
Igf2r表现出双基表达,这表明猪缺乏基特异性调节,因此需要进一步调查Airn在发育中的作用.

关键词:

美国国际航空公司 (AIRNN)在 IGF2R 里面,你会看到 IGF2R.不同地甲基化区域的区别.基因组印记是基因组的印记.在ncRNA中,我们可以看到猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪

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