相关实验视频

Updated: Jul 11, 2025

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

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用于识别G蛋白结合受体的生理相关结合蛋白的协议

Bill X Huang1, Hee-Yong Kim1

  • 1Laboratory of Molecular Signaling, National Institute on Alcohol Abuse and Alcoholism, NIH, 5625 Fishers Lane, Rockville, MD 20852, USA.

STAR protocols
|November 5, 2023
PubMed
概括

这项研究引入了一种新的协议,用于识别G蛋白结合受体 (GPCR) GPR110.0.的结合蛋白. 该方法使用先进的质谱法来寻找细胞表面与GPCR相互作用的蛋白质.

关键词:
质谱测量质量谱测量蛋白质的表达和净化过程蛋白质组学是指蛋白质组学.

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G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
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Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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