对BioFire JI小组的多中心评估与急性骨关节感染的微生物学诊断的改进有关
Llanos Salar-Vidal1, Catarina Chaves2, Ileana T Dianzo-Delgado3
1Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain; CIBERINFEC-CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain.
International journal of medical microbiology : IJMM
|November 5, 2023
概括
生物火联合感染面板多重PCR测试为疑似急性骨关节感染 (OI) 提供了快速诊断. 与传统培养相比,这种分子方法提高了病原体检测,有助于更快的治疗决策.
科学领域:
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断
- 整形外科 整形外科 整形外科
背景情况:
- 准确的微生物学诊断对于有效治疗骨关节感染 (OI) 是必不可少的.
- 传统的突液培养可能很慢,可能会错过苛刻的有机体.
研究的目的:
- 评估BioFire联合感染面板多重PCR测试的性能,用于诊断急性OI.
- 为了将多重PCR结果与传统的突液培养进行比较.
主要方法:
- 对262个来自疑似急性OI患者的突液样本进行了前性多中心研究.
- 使用BioFire联合感染面板多重PCR测试进行分析.
- 将PCR结果与传统微生物培养物的比较.
主要成果:
- 检测到136种微生物;14个样本与培养相比,PCR显示了额外的阳性结果.
- 在单微生物感染中,69%的基因与培养的一致性 (n=87).
- 通过PCR成功地发现了像K. kingae和N. gonorrhoeae这样的棘手生物.
结论:
- 多重复合PCR是一种快速,自动化和有价值的工具,用于诊断急性OI.
- 这种技术可以改善病原体的检测,特别是难以培养的微生物.
- 通过多重PCR进行早期和准确的诊断可以指导及时和适当的患者治疗.
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