带有抗原的鞭状细菌通过皮内注射来增强自适应性免疫反应
Feng Tao1, Qingsong Ye2, Yimiao Chen2
1Department of Andrology, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing 210093, China; State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University, Nanjing University, Nanjing 210093, China.
概括
带有抗原的鞭状细菌 (FB-Ag) 克服皮肤障碍,以提高疫苗的输送. 这种活跃的传递系统改善了树突细胞的激活,提高了治疗疗效和对黑色素瘤和COVID-19的免疫记忆.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 疫苗开发 疫苗开发
背景情况:
- 皮肤的树突细胞对于免疫反应至关重要,但受到皮肤内疫苗分布的限制.
- 目前的皮肤内疫苗策略难以充分利用皮肤内树突细胞来实现最佳的免疫激活.
研究的目的:
- 开发一种使用鞭状细菌来克服皮肤障碍的活性抗原递送系统.
- 增强树突细胞激活和提高皮内疫苗的疗效.
主要方法:
- 通过将抗原加载到性聚合物修饰的表面上,工程化鞭状细菌 (FB-Ag).
- 在体外皮肤模型中评估了FB-Ag运动性和树突细胞激活.
- 在黑色素瘤和对COVID-19抗原的免疫反应的小鼠模型中评估了体内治疗疗效.
主要成果:
- 在实验室中,FB-Ag表现出快速运动性 (∼0.2 μm s-1) 和强大的树突细胞激活.
- 在体内,FB-Ag在皮肤中积极传播抗原,增加与树突细胞的接触并增强激活.
- 在黑色素瘤模型中,FB-Ag显著提高了治疗效果,并诱导了长期的免疫记忆.
- 在小鼠中,FB-Ag提高了COVID-19特定抗体水平和记忆B细胞计数.
结论:
- FB-Ag的活跃运动克服了皮内限制,增强了树突细胞的激活.
- 这种方法为开发更有效的皮内疫苗提供了一个新的策略.
- FB-Ag显示了改善癌症免疫疗法和传染病疫苗反应的潜力.
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