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相关概念视频

Recycling Endosomes and Transcytosis00:58

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The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
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In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
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Regulation of the Unfolded Protein Response01:31

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Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
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The Citric Acid Cycle: Output01:28

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The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
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The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
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Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
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Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
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相关实验视频

Updated: Jul 11, 2025

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
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The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

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IST1规范了选择的回收途径.

Amy K Clippinger1, Teresa V Naismith1, Wonjin Yoo2

  • 1Department of Cell Biology and Physiology, Washington University in St. Louis, St. Louis, Missouri, USA.

Traffic (Copenhagen, Denmark)
|November 5, 2023
PubMed
概括
此摘要是机器生成的。

运输所需的内体组分复合体 (ESCRT) 机器,特别是IST1,对于内体贩运至关重要. IST1调节了从早期内分泌体的关键受体的循环,影响细胞运输通路.

关键词:
AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1克拉斯林是什么意思 克拉斯林是什么意思运输所需的内体分类复杂物.排序 Nexin 的排序转移素受体的受体是转移素的受体.

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The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
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Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
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Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

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相关实验视频

Last Updated: Jul 11, 2025

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
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The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

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The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
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Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 膜贩卖 膜贩卖 膜贩卖 膜贩卖

背景情况:

  • 运输所需的内体组分复合体 (ESCRT) 蛋白质介导膜重塑,包括内囊泡 (ILV) 的形成.
  • 一种ESCRT-III蛋白质的IST1涉及到ILV形成之外的各种内体细胞重塑事件.

研究的目的:

  • 调查IST1及其结合性伙伴CHMP1B在早期内分体贩运中的作用.
  • 阐明IST1与排序素SNX15在调节内体循环通路中的相互作用.

主要方法:

  • 耗尽研究来评估IST1功能.
  • 活细胞显微镜分析蛋白质定位和招募动态.
  • 对内分泌体上的蛋白相互作用的动态和空间分析.

主要成果:

  • IST1和CHMP1B对于早期内体体载体的分裂至关重要.
  • IST1 枯竭会损害转移素受体和马诺6酸盐受体从早期内分泌体的输出.
  • IST1与SNX15相互作用,SNX15与SNX15相互作用,SNX15与SNX15相互作用,SNX15与SNX15相互作用,SNX15与SNX15相互作用,SNX15与SNX15相互作用,SNX15与SNX15相互作用,SNX15与SNX15相互作用,SNX15与SNX15相互作用.

结论:

  • IST1在源自早期/排序内分泌体的特定循环途径中发挥着重要作用.
  • 这种SNX15-IST1相互作用对于调节内体货物分类和贩运至关重要.
  • 这些发现揭示了IST1在内体循环中的新功能.